Crystallization of a globular protein in lipid cubic phase

被引：18
作者：
Tanaka, S [1 ]
Egelhaaf, SU [1 ]
Poon, WCK [1 ]
机构：
[1] Univ Edinburgh, Sch Phys, JCMB, Edinburgh EH9 3JZ, Midlothian, Scotland
来源：
PHYSICAL REVIEW LETTERS | 2004年 / 92卷 / 12期
基金：
英国工程与自然科学研究理事会;
关键词：
D O I：
10.1103/PhysRevLett.92.128102
中图分类号：
O4 [物理学];
学科分类号：
0702 ;
摘要：
We studied the crystallization of a globular protein, lysozyme, in the cubic phase of the lipid mono-olein. The solubility of lysozyme in salt solution decreased by a factor of similar to4 when confined in cubic phase. Monte Carlo simulations and calculations show that this can be explained by the confinement of lysozyme molecules to the narrow water cells in the cubic phase.
引用
收藏
页码：128102 / 1
页数：4
相关论文
共 50 条
[21]   Sans Studies of Bacteriorhodopsin Incorporation and Crystallization in Cubic Phase [J].
Cleveland, Thomas E. ;
Butler, Paul .
BIOPHYSICAL JOURNAL, 2016, 110 (03) :61A-61A
[22]   Lipidic cubic phases as matrices for membrane protein crystallization [J].
Nollert, P .
METHODS, 2004, 34 (03) :348-353
[23]   GPCR Crystallization Using Lipidic Cubic Phase Technique [J].
Yin, Xin ;
Xu, Henghao ;
Hanson, Michael ;
Liu, Wei .
CURRENT PHARMACEUTICAL BIOTECHNOLOGY, 2014, 15 (10) :971-979
[24]   Encapsulation of hemoglobin in a bicontinuous cubic phase lipid [J].
Leslie, SB ;
Puvvada, S ;
Ratna, BR ;
Rudolph, AS .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1996, 1285 (02) :246-254
[25]   Crystallization in lipidic cubic phase. The impact of additives on phase behaviour [J].
Preu, Julia ;
Thielmann, Yvonne ;
Michel, Hartmut .
ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES, 2011, 67 :C468-C468
[26]   CUBIC LIPID-PROTEIN-WATER PHASES [J].
BUCHHEIM, W ;
LARSSON, K .
JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1987, 117 (02) :582-583
[27]   Predicting the Orientation of Lipid Cubic Phase Films [J].
Richardson, Samuel J. ;
Staniec, Paul A. ;
Newby, Gemma E. ;
Terrill, Nick J. ;
Elliott, Joanne M. ;
Squires, Adam M. ;
Gozdz, Wojciech T. .
LANGMUIR, 2014, 30 (45) :13510-13515
[28]   Crystallization of globular proteins [J].
Poon, WCK .
PHYSICAL REVIEW E, 1997, 55 (03) :3762-3764
[29]   Effect of pH on phase separation of globular protein [J].
Sang Gon Kim ;
Young Chan Bae ;
Jae-Jun Kim .
Korean Journal of Chemical Engineering, 2009, 26 :742-747
[30]   Effect of pH on phase separation of globular protein [J].
Kim, Sang Gon ;
Bae, Young Chan ;
Kim, Jae-Jun .
KOREAN JOURNAL OF CHEMICAL ENGINEERING, 2009, 26 (03) :742-747
12345