Long-Term Nitric Oxide Exposure Enhances Lung Cancer Cell Migration

被引:36
作者
Sanuphan, Arpasinee
Chunhacha, Preedakorn
Pongrakhananon, Varisa
Chanvorachote, Pithi [1 ]
机构
[1] Chulalongkorn Univ, Dept Physiol & Pharmacol, Fac Pharmaceut Sci, Bangkok, Thailand
关键词
FOCAL ADHESION KINASE; ANOIKIS RESISTANCE; UP-REGULATION; CAVEOLIN-1; RHO; EXPRESSION; GTPASES; RAC; MICROENVIRONMENT; TRANSFORMATION;
D O I
10.1155/2013/186972
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Nitric oxide (NO) found in the vicinity of lung cancer cells may play a role in the regulation of cancer cell behaviors. To explore the possible effects of NO on cell motility, human lung cancer cells were exposed to nontoxic concentrations of NO for 0-14 days, and the migratory characteristics of the cells were determined. The present study found that long-term treatment with NO significantly enhanced cell migration in a dose- and time-dependent manner. Furthermore, we found that the increased migratory action was associated with the increased expression of caveolin-1 (Cav-1), which in turn activated the focal adhesion kinase (FAK) and ATP-dependent tyrosine kinase (Akt) pathways. Notably, the NO-treated cells exhibited an increased number of filopodia per cell, as well as an increase in the levels of cell division cycle 42 (Cdc42) protein. Together, these results indicate that extended NO exposure has a novel effect on cell migration through a Cav-1-dependent mechanism, a finding that strengthens our understanding of cancer biology.
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页数:9
相关论文
共 39 条
[1]  
Allen WE, 1997, J CELL SCI, V110, P707
[2]  
[Anonymous], 2009, CURR CHEM BIOL, DOI DOI 10.2174/187231309787158271
[3]  
Cary LA, 1996, J CELL SCI, V109, P1787
[4]   Nitric Oxide Regulates Lung Carcinoma Cell Anoikis through Inhibition of Ubiquitin-Proteasomal Degradation of Caveolin-1 [J].
Chanvorachote, Pithi ;
Nimmannit, Ubonthip ;
Lu, Yongju ;
Talbott, Siera ;
Jiang, Bing-Hua ;
Rojanasakul, Yon .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (41) :28476-28484
[5]   Nitric oxide influences injury-induced microglial migration and accumulation in the leech CNS [J].
Chen, AL ;
Kumar, SM ;
Sahley, CL ;
Muller, KJ .
JOURNAL OF NEUROSCIENCE, 2000, 20 (03) :1036-1043
[6]  
Chunhacha Preedakorn, 2012, Int J Physiol Pathophysiol Pharmacol, V4, P149
[7]   Caveolin-1 regulates Mcl-1 stability and anoikis in lung carcinoma cells [J].
Chunhacha, Preedakorn ;
Pongrakhananon, Varisa ;
Rojanasakul, Yon ;
Chanvorachote, Pithi .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2012, 302 (09) :C1284-C1292
[8]  
Dhar A, 2003, MOL CANCER THER, V2, P1285
[9]   High levels of oxidative stress in patients with advanced lung cancer [J].
Esme, Hidir ;
Cemek, Mustafa ;
Sezer, Murat ;
Saglam, Hayrettin ;
Demir, Adalet ;
Melek, Huseyin ;
Unlu, Mehmet .
RESPIROLOGY, 2008, 13 (01) :112-116
[10]   The role of nitric oxide in tumour progression [J].
Fukumura, Dai ;
Kashiwagi, Satoshi ;
Jain, Rakesh K. .
NATURE REVIEWS CANCER, 2006, 6 (07) :521-534