RhoE enhances multidrug resistance of gastric cancer cells by suppressing Bax

被引：27
作者：
Li, Kai
Lu, Yuanyuan
Liang, Jie
Luo, Guanhong
Ren, Gui
Wang, Xin [1 ]
Fan, Daiming
机构：
[1] Fourth Mil Med Univ, State Key Lab Canc Biol, Xian 710032, Shaanxi, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 379卷 / 02期
关键词：
Multidrug resistance; Gastric cancer; RhoE; Apoptosis; Bax; DIFFERENTIAL DISPLAY; INDUCED APOPTOSIS; P-GLYCOPROTEIN; ROCK-I; GENE; EXPRESSION; PROTEIN; PROGRESSION; SURVIVAL; LINES;
D O I：
10.1016/j.bbrc.2008.12.044
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have previously reported that RhoE is overexpressed in the SGC7901/VCR cell line. However, the potential role of RhoE in the development of multidrug resistance of gastric cancer is unknown. In the present study, RhoE enhanced the resistance of SGC7901 cells to several kinds of antitumor drugs. RhoE overexpression did not alter the intracellular adriamycin accumulation of SGC7901 cells nor the expression of P-gp and MRP-1, but protected SGC7901 cells from vincristine-induced apoptosis. RhoE was found to downregulate the expression of Bax at a posttranscriptional level. Western blot revealed no effects of RhoE on the activities of the Caspase family of proteins. in brief, our study demonstrated that RhoE may promote the multidrug resistance phenotype of gastric cancer cells by decreasing the expression of Bax at posttranscriptional level, thus inhibiting vincristine-induced apoptosis. (C) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码：212 / 216
页数：5
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