Linking hydrolytic activities to variables influencing a submerged membrane bioreactor (MBR) treating urban wastewater under real operating conditions

被引：19

作者：

Gomez-Silvan, C. ^{[1
]}

Arevalo, J. ^{[2
]}

Perez, J. ^{[2
]}

Gonzalez-Lopez, J. ^{[1
]}

Rodelas, B. ^{[1
]}

机构：

[1] Fac Farm, Dept Microbiol, Granada 18071, Spain

[2] Univ Granada, Dept Ingn Civil, E-18071 Granada, Spain

来源：

WATER RESEARCH | 2013年 / 47卷 / 01期

关键词：

Wastewater treatment; Hydrolytic enzymes; Phosphatase; Glucosidase; Protease; Multivariate analysis; ACTIVATED-SLUDGE SYSTEM; ENZYME-ACTIVITIES; COMMUNITY STRUCTURE; MICROBIAL COMMUNITIES; COUPLED BIOREACTORS; AEROBIC TREATMENT; ULTRAFILTRATION; SEDIMENTS; SOLIDS; SOIL;

D O I：

10.1016/j.watres.2012.09.032

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The seasonal variation of the hydrolytic activities acid and alkaline phosphatase, glucosidase and protease, was studied in both the aerated and anoxic phases of a full-scale membrane bioreactor (MBR) (total operational volume = 28.2 m(3)), operated in predenitrification mode and fed real urban wastewater. Non-metric multidimensional scaling (MDS) and BIO-ENV analysis were used to study the distribution of enzyme activities in different seasons of the year (spring, summer and autumn) and unveil their relationships with changes in variables influencing the system (composition of influent wastewater, activated sludge temperature and biomass concentration in the bioreactors). The activities of all the tested hydrolases were remarkably dynamic, and each enzyme showed complex and diverse patterns of variation. Except in the summer season, the variables included in this study gave a good explanation of those patterns and displayed high and consistent correlations with them; however, markedly different correlation trends were found in each season, indicating dissimilar adaptation responses of the community to the influence of changing conditions. A consistent and highly negative correlation between protease and alpha-glucosidase was revealed in all the experiments. The variables included in this study showed contrary influences on these activities, suggesting an alternation of the major groups of carbon-degrading hydrolases in connection to changes in temperature and the availability and composition of nutrients in the different seasons. Sampling over a long period of time was required to adequately lay down the links between hydrolytic activities and the variables influencing the MBR system. These results highlight the complexity of the regulation of substrate degradation by the mixed microbial sludge communities under real operating conditions. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：66 / 78

页数：13

共 45 条

[1]

AEMeT, 2005, SUMM CLIM GUID SPAIN

[2]

Bitton G., 2010, Wastewater Microbiology, VFourth

[3] CHARACTERIZATION OF ENZYME-ACTIVITY IN ACTIVATED-SLUDGE USING RAPID ANALYSES FOR SPECIFIC HYDROLASES [J].