Blind-deconvolution optical-resolution photoacoustic microscopy in vivo

被引：95

作者：

Chen, Jianhua ^{[1
]}

Lin, Riqiang ^{[1
]}

Wang, Huina ^{[1
]}

Meng, Jing ^{[1
]}

Zheng, Hairong ^{[2
]}

Song, Liang ^{[1
]}

机构：

[1] Chinese Acad Sci, Shenzhen Inst Adv Technol, Inst Biomed & Hlth Engn, Res Lab Biomed Opt & Mol Imaging, Shenzhen 518055, Peoples R China

[2] Chinese Acad Sci, Shenzhen Inst Adv Technol, Inst Biomed & Hlth Engn, Paul C Lauterbur Res Ctr Biomed Imaging, Shenzhen 518055, Peoples R China

来源：

OPTICS EXPRESS | 2013年 / 21卷 / 06期

基金：

中国国家自然科学基金;

关键词：

COMPUTED-TOMOGRAPHY;

D O I：

10.1364/OE.21.007316

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Optical-resolution photoacoustic microscopy (OR-PAM) is becoming a vital tool for studying the microcirculation system in vivo. By increasing the numerical aperture of optical focusing, the lateral resolution of OR-PAM can be improved; however, the depth of focus and thus the imaging range will be sacrificed correspondingly. In this work, we report our development of blind-deconvolution optical-resolution photoacoustic microscopy (BD-PAM) that can provide a lateral resolution similar to 2-fold finer than that of conventional OR-PAM (3.04 vs. 5.78 mu m), without physically increasing the system's numerical aperture. The improvement achieved with BD-PAM is demonstrated by imaging graphene nanoparticles and the microvasculature of mice ears in vivo. Our results suggest that BD-PAM may become a valuable tool for many biomedical applications that require both fine spatial resolution and extended depth of focus. (C) 2013 Optical Society of America

引用

页码：7316 / 7327

页数：12

共 40 条

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