An alginate-layer technique for culture of Brassica oleracea L. protoplasts

被引：39

作者：

Kielkowska, Agnieszka ^{[1
]}

Adamus, Adela ^{[1
]}

机构：

[1] Univ Agr Krakow, Dept Genet Plant Breeding & Seed Sci, PL-31425 Krakow, Poland

来源：

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT | 2012年 / 48卷 / 02期

关键词：

Brassica alba; rubra; sabauda; Calcium alginate; Hypocotyl; Mesophyll; DIRECT DNA UPTAKE; PLANT-REGENERATION; LEAF PROTOPLASTS; HYPOCOTYL PROTOPLASTS; MESOPHYLL PROTOPLASTS; ARABIDOPSIS-THALIANA; SOMATIC HYBRIDS; NAPUS L; GENETIC-TRANSFORMATION; EFFICIENT REGENERATION;

D O I：

10.1007/s11627-012-9431-6

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Ten accessions belonging to the Brassica oleracea subspecies alba and rubra, and to B. oleracea var. sabauda were used in this study. Protoplasts were isolated from leaves and hypocotyls of in vitro grown plants. The influence of selected factors on the yield, viability, and mitotic activity of protoplasts immobilized in calcium alginate layers was investigated. The efficiency of protoplast isolation from hypocotyls was lower (0.7 +/- 0.1 x 10(6) ml(-1)) than for protoplasts isolated from leaf mesophyll tissue (2 +/- 0.1 x 10(6) ml(-1)). High (70-90%) viabilities of immobilized protoplasts were recorded, independent of the explant sources. The highest proportion of protoplasts undergoing divisions was noted for cv. Reball F1, both from mesophyll (29.8 +/- 2.2%) and hypocotyl (17.5 +/- 0.3%) tissues. Developed colonies of callus tissue were subjected to regeneration and as a result plants from six accessions were obtained.

引用

页码：265 / 273

页数：9

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