Optimization of the CDC Protocol of Molecular Diagnosis of COVID-19 for Timely Diagnosis

被引：11

作者：

Chen, Chao-Ju ^{[1
]}

Hsieh, Li-Ling ^{[1
]}

Lin, Shu-Kai ^{[1
]}

Wang, Chu-Feng ^{[1
]}

Huang, Yi-Hui ^{[1
]}

Lin, Shang-Yi ^{[1
,2
,3
]}

Lu, Po-Liang ^{[1
,2
,3
]}

机构：

[1] Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Dept Lab Med, Kaohsiung 80756, Taiwan

[2] Kaohsiung Med Univ Hosp, Dept Internal Med, Kaohsiung 80756, Taiwan

[3] Kaohsiung Med Univ, Coll Med, Kaohsiung 80708, Taiwan

来源：

DIAGNOSTICS | 2020年 / 10卷 / 05期

关键词：

coronavirus disease 2019 (COVID-19); severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); molecular diagnostics; real-time reverse transcriptase polymerase chain (RT-PCR); BOVINE SERUM-ALBUMIN;

D O I：

10.3390/diagnostics10050333

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Coronavirus disease 2019 (COVID-19), the current uncontrolled outbreak of infectious disease, has caused significant challenges throughout the world. A reliable rapid diagnostic test for COVID-19 is demanded worldwide. The real-time reverse transcriptase polymerase chain was one of the most quickly established methods in the novel viral pandemic and was considered as the gold standard for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this report, we illustrate our experience of applying a protocol from the Taiwan CDC and achieving assay optimization in the immediate circumstances to meet the urgent medical and public health needs.

引用

页数：6

共 10 条

[1] Novel human coronavirus (SARS-CoV-2): A lesson from animal coronaviruses [J].

Decaro, Nicola ;

Lorusso, Alessio .

VETERINARY MICROBIOLOGY, 2020, 244

[2] The species Severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2 [J].

Gorbalenya, Alexander E. ;

Baker, Susan C. ;

Baric, Ralph S. ;

de Groot, Raoul J. ;

Drosten, Christian ;

Gulyaeva, Anastasia A. ;

Haagmans, Bart L. ;

Lauber, Chris ;

Leontovich, Andrey M. ;

Neuman, Benjamin W. ;

Penzar, Dmitry ;

Perlman, Stanley ;

Poon, Leo L. M. ;

Samborskiy, Dmitry V. ;

Sidorov, Igor A. ;

Sola, Isabel ;

Ziebuhr, John .

NATURE MICROBIOLOGY, 2020, 5 (04) :536-544

[3]

MAHASE E, 2020, BMJ-BRIT MED J, V368, DOI [DOI 10.1136/BMJ.M408, 10.1136/bmj.m408]

[4]

Nagai M, 1998, BIOCHEM MOL BIOL INT, V44, P157

[5]

Nitsche A, 1999, CLIN CHEM, V45, P1932

[6] The use of bovine serum albumin to improve the RT-qPCR detection of foodborne viruses rinsed from vegetable surfaces [J].

Plante, D. ;

Belanger, G. ;

Leblanc, D. ;

Ward, P. ;

Houde, A. ;

Trottier, Y. -L. .

LETTERS IN APPLIED MICROBIOLOGY, 2011, 52 (03) :239-244

[7]

Taiwan CDC, 2019 NCOV VIR NUCL A

[8]

World Health Organization, 2020, situation Report-51, DOI [10.1001/jama.2020.2633, DOI 10.1001/JAMA.2020.2633]

[9] Point-of-Care RNA-Based Diagnostic Device for COVID-19 [J].

Yang, Ting ;

Wang, Yung-Chih ;

Shen, Ching-Fen ;

Cheng, Chao-Min .

DIAGNOSTICS, 2020, 10 (03)

[10]

Zhu N, 2020, NEW ENGL J MED, V382, P727, DOI [10.1056/NEJMoa2001017, 10.1172/JCI89857]

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