Platelet lysate and adipose mesenchymal stromal cells on silk fibroin nonwoven mats for wound healing

被引：13
作者：
Theodora, Chlapanidas [1 ]
Sara, Perteghella [1 ]
Silvio, Farago [2 ]
Alessandra, Boschi [2 ]
Giuseppe, Tripodo [1 ]
Barbara, Vigani [1 ]
Barbara, Crivelli [1 ]
Sabrina, Renzi [3 ]
Silvia, Dotti [3 ]
Stefania, Preda [4 ]
Mario, Marazzi [5 ]
Luisa, Torre Maria [1 ]
Maura, Ferrari [3 ]
机构：
[1] Univ Pavia, Med Chem & Technol Sect, Dept Drug Sci, I-27100 Pavia, Italy
[2] Innovhub, Stn Sperimentali Ind, Silk Div, I-20133 Milan, Italy
[3] Ist Zooprofilatt Sperimentale Lombardia & Emilia, Cell Culture Ctr, I-25124 Brescia, Italy
[4] Univ Pavia, Pharmacol Sect, Dept Drug Sci, I-27100 Pavia, Italy
[5] Osped Niguarda Ca Granda, Struttura Semplice Tissue Therapy, I-20162 Milan, Italy
来源：
JOURNAL OF APPLIED POLYMER SCIENCE | 2016年 / 133卷 / 05期
关键词：
biomaterials; fibers; properties and characterization; HUMAN BONE-MARROW; STEM-CELLS; IN-VITRO; VASCULAR FRACTION; COMPOSITE NANOFIBERS; HYALURONIC-ACID; TISSUE; SCAFFOLDS; REGENERATION; DELIVERY;
D O I：
10.1002/app.42942
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
In this work platelet lysate (PL) and adipose-derived mesenchymal stromal cells (ASCs) seeded on nonwoven fibroin mats were in vitro and in vivo evaluated for tissue regenerative applications. Nonwoven mats obtained by a large scale water entanglement technique were characterized for their physico-chemical properties. Results indicated a high purity of fibroin fibers, their stability after sterilization process and appropriate technological properties suitable for tissue engineering. Moreover, the scaffolds in vitro supported adhesion and migration of ASCs and the presence of PL improved the cell proliferation. The products were then applied on epithelial/dermal wounds carried out on the dorsal surface of rabbit: the skin reparative process was solved in 9 days, with a completely restitutio ad integrum of the epithelium in animals treated with PL alone; ASCs did not further improve the wound healing. (c) 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 42942.
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页数：13
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