Targeted immobilization of titanium (IV) on magnetic mesoporous nanomaterials derived from metal-organic frameworks for high-efficiency phosphopeptide enrichment in biological samples

被引：18

作者：

Pu, Chenlu ^{[1
]}

Zhao, Hongli ^{[1
]}

Gu, Qinying ^{[1
]}

Zheng, Yu ^{[1
]}

Lan, Minbo ^{[1
,2
]}

机构：

[1] East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

[2] East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

来源：

MICROCHIMICA ACTA | 2020年 / 187卷 / 10期

基金：

上海市自然科学基金;

关键词：

Metal-organic frameworks; Mesoporous structure; Selective modification; Phosphopeptides enrichment; Mass spectrometry; SELECTIVE ENRICHMENT; TI4+; MICROSPHERES;

D O I：

10.1007/s00604-020-04556-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A selectively modified porous metal/carbon nanocomposite was fabricated to enhance the enrichment of low-abundance phosphopeptides from biological samples. The carbon matrix derived from the metal-organic framework provides a suitable pore size to allow the diffusion of peptides, while the deliberately modified metal nanoparticles within the pores enhance their interaction with the phosphopeptides. This nanocomposite shows extremely high enrichment selectivity for phosphopeptides in the MALDI-TOF MS detection, even when the molar ratio of alpha-casein digests versus bovine serum albumin digests was up to about 1:20,000. By combining such nanocomposite with nano-LC-MS/MS, 4556 unique phosphopeptides were identified with high selectivity (95.2%) from HeLa cell extracts. Furthermore, phosphopeptides from prostate tissue digests were also determined. A total of 277 and 1242 phosphopeptides were identified from normal and tumor tissues of a patient with prostate cancer, respectively. This indicates that phosphorylation and prostate cancer can be related to each other.

引用

页数：9

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