Changes in Allergenicity of Porcine Serum Albumin by Microwave, Sonication, and High Hydrostatic Pressure

Even though pork have frequently induced allergic reactions in Korea, few papers have been published on pork allergy. This study was carried out to investigate the changes allergenicity of porcine serum albumin (PSA) by microwave, sonication, and high hydrostatic pressure (HHP). The binding ability of p-IgG to PSA treated with microwave (1, 5, or 10 min) directly decreased will increasing, treatment time. Particularly, the binding ability of PSA treated 10 min was about 30%. Immunoblotting assay with p-IgG showed that band of PSA treated microwave directly disappeared at 5 and 10 min. However. the binding ability of PSA was not chanted by the microwave treatment without heat. Also the reduction of allergenicity by sonication or HHP treatment was not found. In conclusion, allergenicity of PSA treated with microwave directly decreased with increasing time, therefore these results may be used for development of hypoallergenic pork.