Seminal plasma microRNAs: potential biomarkers for spermatogenesis status

被引：125

作者：

Wu, Wei ^{[1
,2
]}

Hu, Zhibin ^{[3
]}

Qin, Yufeng ^{[1
,2
]}

Dong, Jing ^{[3
]}

Dai, Juncheng ^{[3
]}

Lu, Chuncheng ^{[1
,2
]}

Zhang, Wei ^{[1
,4
]}

Shen, Hongbing ^{[3
]}

Xia, Yankai ^{[1
,2
]}

Wang, Xinru ^{[1
,2
]}

机构：

[1] Nanjing Med Univ, State Key Lab Reprod Med, Inst Toxicol, Sch Publ Hlth, Nanjing 210029, Jiangsu, Peoples R China

[2] Nanjing Med Univ, Key Lab Modern Toxicol, Minist Educ, Nanjing 210029, Jiangsu, Peoples R China

[3] Nanjing Med Univ, Ctr Canc, Dept Epidemiol & Biostat, Nanjing 210029, Jiangsu, Peoples R China

[4] Nanjing Med Univ, Affiliated Hosp 1, Dept Urol, Nanjing 210029, Jiangsu, Peoples R China

来源：

MOLECULAR HUMAN REPRODUCTION | 2012年 / 18卷 / 10期

基金：

中国国家自然科学基金;

关键词：

microRNA; seminal plasma; azoospermia; oligozoospermia; male infertility; MESSENGER-RNA; EXPRESSION; SERUM; BIOGENESIS; DIAGNOSIS; PROFILES; GENOMICS; CLUSTER; CANCER; MIRNA;

D O I：

10.1093/molehr/gas022

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

MicroRNAs (miRNAs) are small non-coding RNAs (1825 nt), playing important regulatory roles via interaction with cellular messenger RNAs. The altered expression of miRNAs in specific tissues has been associated with diseases such as cancer and diabetes. We examined the presence of two selected miRNAs (miR-19b and let-7a) in human seminal plasma from fertile men and idiopathic infertile patients with oligozoospermia and non-obstructive azoospermia (NOA) using quantitative real-time PCR. We detected miRNAs in the seminal plasma of humans. The levels of miRNAs in the seminal plasma were reproducible in repeat samples from the same individuals. In addition, we examined the expression patterns of two selected miRNAs in 96 idiopathic infertile males (48 oligozoospermia and 48 NOA) and 48 fertile controls. Another 48 individuals of each group were used for verification. Our data showed that the expression levels of these two miRNAs in the seminal plasma significantly increased in idiopathic infertile males with NOA compared with fertile controls, whereas the expression levels were similar between idiopathic infertile males with oligozoospermia and fertile controls. In conclusion our results indicate that the expression of miR-19b and let-7a in the seminal plasma are reproducible and stable. Aberrant over-expression levels of miR-19b and let-7a may be an indicator of spermatogenic failure.

引用

页码：489 / 497

页数：9

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