Ultra-sensitive detection of malathion using quantum dots-polymer based fluorescence aptasensor

被引：79

作者：

Bala, Rajni ^{[1
,2
]}

Swami, Anuradha ^{[3
]}

Tabujew, Ilja ^{[4
]}

Peneva, Kalina ^{[4
]}

Wangoo, Nishima ^{[3
]}

Sharma, Rohit K. ^{[1
,2
]}

机构：

[1] Panjab Univ, Dept Chem, Sect 14, Chandigarh 160014, India

[2] Panjab Univ, Ctr Adv Studies Chem, Sect 14, Chandigarh 160014, India

[3] Panjab Univ, UIET, Dept Appl Sci, Sect 25, Chandigarh 160014, India

[4] Friedrich Schiller Univ Jena, Jena Ctr Soft Matter, Inst Organ Chem & Macromol Chem, Lessingstr 8, D-07743 Jena, Germany

来源：

BIOSENSORS & BIOELECTRONICS | 2018年 / 104卷

关键词：

Core shell quantum dots; Guanidinium-containing polymers; Aptamer; Fluorescence quenching; Malathion; ORGANOPHOSPHORUS PESTICIDE; COLORIMETRIC DETECTION; GOLD NANOPARTICLES; RECOGNITION; BIOSENSOR; MATRICES; ETHYL; SIRNA; ASSAY;

D O I：

10.1016/j.bios.2017.12.034

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A novel detection platform with high malathion specificity has been developed, which operates based on the signal response in the fluorescence of CdTe@CdS quantum dots (QDs). The designed nanoprobe comprises of QDs, poly(N-(3-guanidinopropyl)methacrylamide) homopolymer (PGPMA) and malathion specific aptamer. The interaction of aptamer with malathion results in switching off of the fluorescence signal of the probe due to the availability of the cationic polymer, which causes quenching of the QDs. However, in the absence of malathion, the polymer interacts with the aptamer, via electrostatic interactions thereby rendering the fluorescence of QDs unaffected. The assay exhibited excellent sensitivity towards malathion with a detection limit of 4 pM. A logarithmic correlation was observed in a wide range of malathion concentrations from 0.01 nm to 1 mu M, facilitating the potential of proposed assay in the quantitative determination of the analyte of interest. The selectivity of the designed probe was confirmed in the presence of various pesticides, commonly employed in agricultural fields.

引用

页码：45 / 49

页数：5

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