Effect of Epothilone B on Cell Cycle, Metabolic Activity, and Apoptosis Induction on Human Epithelial Cancer Cells-Under Special Attention of Combined Treatment with Ionizing Radiation

被引：2
作者：
Baumgart, Tonja [1 ]
Kriesen, Stephan [1 ]
Hildebrandt, Guido [1 ]
Manda, Katrin [1 ]
机构：
[1] Univ Rostock, Dept Radiotherapy & Radiat Oncol, D-18059 Rostock, Germany
来源：
CANCER INVESTIGATION | 2012年 / 30卷 / 08期
关键词：
Epothilone B; Irradiation; Cell cycle; Apoptosis; Multinuclear cells; MICROTUBULE-STABILIZING AGENTS; HUMAN TUMOR-CELLS; PROSTATE-CANCER; IN-VITRO; MITOTIC CATASTROPHE; TAXOL; PATUPILONE; PACLITAXEL; TUBULIN; ARREST;
D O I：
10.3109/07357907.2012.716469
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
In recent studies, epothilone B was shown to have a cytotoxic and radiosensitizing effect on cells. The aim of our investigation was to explain this impact by examining the mode of action of epothilone B on FaDu and A549 tumor cells. Flow cytometry was used for cell cycle distribution and for the evaluation of apoptosis. Metabolic activity was studied by proliferation assay. Influence on nuclei morphology was investigated by DNA-staining. We showed that epothilone B-induced G2/M accumulation is the main rationale for drug-induced radiosensitivity. The cytotoxic effect resulted in apoptotic cell death, decreased metabolic activity, and formation of multinucleated cells.
引用
收藏
页码：593 / 603
页数：11
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