Self-Assembly Capillary Coatings of Diazoresin and PEG for CE Analysis of Proteins

被引:5
作者
Liu, Peng [1 ]
Yu, Bing [1 ,2 ]
Cong, Hai-Lin [1 ,2 ]
Tang, Jian-Guo [1 ,2 ]
Sun, Jun-Miao [1 ]
机构
[1] Qingdao Univ, Coll Chem & Environm Engn, Qingdao 266071, Peoples R China
[2] Qingdao Univ, Growing Base State Key Lab, Lab New Fiber Mat & Modern Text, Qingdao 266071, Peoples R China
关键词
Diazoresin; polyethylene glycol; capillary electrophoresis; coating; protein; LAYER; ELECTROPHORESIS;
D O I
10.1080/10584587.2012.687269
中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
A novel method for the preparation of covalently bonded capillary coatings of polyethylene glycol (PEG) was demonstrated. Layer by layer self-assembly film of diazoresin (DR) and PEG based on hydrogen bonding was fabricated on the inner wall of capillary. The hydrogen bonding was converted into covalent bonding after treatment with UV light through the unique photochemistry reaction of DR. The covalently bonded coatings suppressed protein adsorption on the inner surface of the capillary, and a baseline separation of three basic proteins was achieved using capillary electrophoresis (CE).
引用
收藏
页码:61 / 66
页数:6
相关论文
共 14 条
[1]   Non-adhesive PEG hydrogel nanostructures for self-assembly of highly ordered colloids [J].
Cong, Hailin ;
Revzin, Alexander ;
Pan, Tingrui .
NANOTECHNOLOGY, 2009, 20 (07)
[2]   Capillary electrophoresis-mass spectrometry of intact basic proteins using Polybrene-dextran sulfate-Polybrene-coated capillaries: System optimization and performance [J].
Haselberg, Rob ;
de Jong, Gerhardus J. ;
Somsen, Govert W. .
ANALYTICA CHIMICA ACTA, 2010, 678 (01) :128-134
[3]   Fabrication of Magnetic Thin Films by a Layer-by-Layer Self-Assembly Approach [J].
Lin, Weihong ;
Sun, Weilin ;
Yang, Jun ;
Sun, Qihang ;
Shen, Zhiquan .
JOURNAL OF PHYSICAL CHEMISTRY C, 2009, 113 (39) :16884-16895
[4]   Flexible and disposable immunosensors based on layer-by-layer self-assembled carbon nanotubes and biomolecules [J].
Lu, Miao ;
Lee, Dongjin ;
Xue, Wei ;
Cui, Tianhong .
SENSORS AND ACTUATORS A-PHYSICAL, 2009, 150 (02) :280-285
[5]   Multilayer films of cationic graphene-polyelectrolytes and anionic graphene-polyelectrolytes fabricated using layer-by-layer self-assembly [J].
Rani, Adila ;
Oh, Kyoung Ah ;
Koo, Hyeyoung ;
Lee, Hyung Jung ;
Park, Min .
APPLIED SURFACE SCIENCE, 2011, 257 (11) :4982-4989
[6]   Layer-by-Layer Quantum Dot Constructs Using Self-Assembly Methods [J].
Rauf, Sakandar ;
Glidle, Andrew ;
Cooper, Jonathan M. .
LANGMUIR, 2010, 26 (22) :16934-16940
[7]   Layer-by-layer self-assembly of multiwalled carbon nanotube polyelectrolytes prepared by in situ radical polymerization [J].
Shen, Jianfeng ;
Hu, Yizhe ;
Qin, Chen ;
Ye, Mingxin .
LANGMUIR, 2008, 24 (08) :3993-3997
[8]   Preparation of Functional Aptamer Films Using Layer-by-Layer Self-Assembly [J].
Sultan, Yasir ;
Walsh, Ryan ;
Monreal, Carlos ;
DeRosa, Maria C. .
BIOMACROMOLECULES, 2009, 10 (05) :1149-1154
[9]   Layer-by-layer assembly of a conformal nanothin PEG coating for intraportal islet transplantation [J].
Wilson, John T. ;
Cui, Wanxing ;
Chaikof, Elliot L. .
NANO LETTERS, 2008, 8 (07) :1940-1948
[10]   Fabrication and characterization of stable ultrathin film micropatterns containing DNA and photosensitive polymer diazoresin [J].
Yu, B ;
Cong, HL ;
Liu, HW ;
Lu, CH ;
Wei, F ;
Cao, WX .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2006, 384 (02) :385-390