Oxidative stress in growth hormone transgenic coho salmon with compressed lifespan - a model for addressing aging

被引：24

作者：

Almroth, Bethanie Carney ^{[1
]}

Johnsson, Joergen I. ^{[1
]}

Devlin, Robert ^{[2
]}

Sturve, Joachim ^{[1
]}

机构：

[1] Univ Gothenburg, Dept Biol & Environm Sci, SE-40530 Gothenburg, Sweden

[2] Fisheries & Oceans Canada, W Vancouver, BC, Canada

来源：

FREE RADICAL RESEARCH | 2012年 / 46卷 / 10期

关键词：

antioxidants; molecular damage; metabolism; rate of living; fish; growth; transgenic; GH; OXYGEN SPECIES PRODUCTION; FREE-RADICAL THEORY; ONCORHYNCHUS-TSHAWYTSCHA FILLETS; MICROTITER PLATE ASSAY; 15; DEGREES-C; RAINBOW-TROUT; BODY-COMPOSITION; ATLANTIC SALMON; DROSOPHILA-MELANOGASTER; CAENORHABDITIS-ELEGANS;

D O I：

10.3109/10715762.2012.698009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Growth hormone (GH) transgenic fish have dramatically enhanced growth rates, increased oxygen demands and reactive oxygen species production. GH-transgenic coho salmon provide an opportunity to address effects of increased metabolism on physiological aging. The objective of this study was to compare oxidative stress in wild-type (WT) and GH-transgenic (T) coho salmon (Oncorhynchus kisutch) of different ages (1 and 2 years). Antioxidant enzyme activity, protein carbonyls (PC) and glutathione (GSH, GSSG) were measured. PC correlated to growth rates in individual fish. T fish exhibited lower antioxidant enzyme activities and GSH levels compared to the WT, while levels of PC and GSSG were higher. Age affects were observed in both WT and T fish; enzyme activities and GSH decreased while PC and GSSG increased. Our results support the metabolic rate theory of aging. This study aims to be a platform for continued studies of the theories of aging using fish as model organisms.

引用

页码：1183 / 1189

页数：7

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