Rhodamine-based fluorescent off-on sensor for Fe3+ - in aqueous solution and in living cells: 8-aminoquinoline receptor and 2: 1 binding

被引：74

作者：

Huang, Junhai ^{[1
,2
,3
]}

Xu, Yufang ^{[1
,2
]}

Qian, Xuhong ^{[1
,2
]}

机构：

[1] E China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

[2] E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China

[3] Shanghai Inst Pharmaceut Ind, Zhangjiang R&D Ctr, Shanghai 200040, Peoples R China

来源：

DALTON TRANSACTIONS | 2014年 / 43卷 / 16期

基金：

中国国家自然科学基金; 国家高技术研究发展计划(863计划);

关键词：

SELECTIVE DETECTION; ENERGY-TRANSFER; IRON-CHELATORS; ZINC IONS; METAL-ION; CHEMOSENSOR; DESIGN; PROBE; HG2+; CHEMODOSIMETER;

D O I：

10.1039/c3dt53159g

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

8-Aminoquinoline (8-AQ) and 2-aminopyridine (AmPyr) both as ionophoric agents were robustly used in development of a cation sensor. To evaluate the effect of complexation with cations, flexible 2-aminopyridine and rigid 8-AQ moiety were directly introduced into rhodamine chromophore and two candidate fluorescent sensors were constructed successfully. Rh-AQ with a rigid 8-AQ fragment exhibited turn-on fluorescence and color responses to Fe3+ ion over other metal ions related to biology in aqueous solution. The sensor Rh-AmPyr with a flexible 2-aminopyridine did not give positive responses to cations although with a similar binding cavity as on Rh-AQ. Furthermore, the 2 : 1 recognition mode of Rh-AQ with Fe3+ was proved according to the 1D and 2D COSY H-H NMR experiments. The live cell imaging experiments demonstrated that Rh-AQ could be successfully applied as a bioimaging agent for monitoring Fe3+ in living cells.

引用

页码：5983 / 5989

页数：7

共 85 条

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