Gene expressions of ubiquitin and hsp70 following focal ischaemia in rat brain

被引:12
作者
Noga, M
Hayashi, T
Tanaka, J
机构
[1] JIKEI UNIV,DEPT PSYCHIAT,MINATO KU,TOKYO 105,JAPAN
[2] DAIICHI PHARMACEUT CO LTD,TOKYO R&D CTR,EDOGAWA KU,TOKYO,JAPAN
关键词
focal brain ischaemia; Hsp70; mRNA; middle cerebral artery occlusion; ubiquitin mRNA;
D O I
10.1097/00001756-199703240-00036
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
EXPRESSION of genes coding for ubiquitin and heat-shock protein (hsp) 70 were examined by in situ hybridization using a rat model with permanent occlusion of the distal middle cerebral artery (MCA). Only polyubiquitin (UbC) mRNA increased markedly following ischaemia in the central zone of the MCA territory of the neocortex. UbC gene expression reached the maximum level 4 h post-occlusion and remained elevated at 24 h. UbC expression was retarded slightly compared with that of the hsp70 gene. UbB and Ub-S30 were expressed at almost similar levels in both the ischaemic and non-ischaemic hemispheres. These results indicated that UbC probably has the most stress-inducible characteristics among the three ubiquitin genes.
引用
收藏
页码:1239 / 1241
页数:3
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