Neuromuscular Plasticity in a Mouse Neurotoxic Model of Spinal Motoneuronal Loss

被引:27
作者
Gulino, Rosario [1 ]
Vicario, Nunzio [2 ]
Giunta, Maria A. S. [2 ]
Spoto, Graziana [2 ]
Calabrese, Giovanna [2 ]
Vecchio, Michele [3 ,4 ]
Gulisano, Massimo [5 ]
Leanza, Giampiero [6 ]
Parenti, Rosalba [2 ]
机构
[1] Univ Catania, Sect Physiol, Dept Biomed & Biotechnol Sci, Lab Neurophysiol, I-95123 Catania, Italy
[2] Univ Catania, Sect Physiol, Dept Biomed & Biotechnol Sci, Lab Cellular & Mol Physiol, I-95123 Catania, Italy
[3] Univ Catania, AOU Policlin Vittorio Emanuele, Rehabil Unit, I-95123 Catania, Italy
[4] Univ Catania, Sect Pharmacol, Dept Biomed & Biotechnol Sci, I-95123 Catania, Italy
[5] Univ Catania, Dept Drug Sci, Lab Synthet & Syst Biol, I-95125 Catania, Italy
[6] Univ Catania, Dept Drug Sci, Lab Neurogenesis & Repair, I-95125 Catania, Italy
关键词
neurodegeneration; spinal cord; gastrocnemius muscle; CTB-Saporin; neuronal plasticity; AMPA receptor; motoneuron; astrocyte; AMYOTROPHIC-LATERAL-SCLEROSIS; RETICULARIS TEGMENTI PONTIS; SYNAPTIC PLASTICITY; SKELETAL-MUSCLE; SONIC HEDGEHOG; DISEASE PROGRESSION; LUMBAR MOTONEURONS; SOD1; MICE; CORD; PROJECTIONS;
D O I
10.3390/ijms20061500
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Despite the relevant research efforts, the causes of amyotrophic lateral sclerosis (ALS) are still unknown and no effective cure is available. Many authors suggest that ALS is a multi-system disease caused by a network failure instead of a cell-autonomous pathology restricted to motoneurons. Although motoneuronal loss is the critical hallmark of ALS given their specific vulnerability, other cell populations, including muscle and glial cells, are involved in disease onset and progression, but unraveling their specific role and crosstalk requires further investigation. In particular, little is known about the plastic changes of the degenerating motor system. These spontaneous compensatory processes are unable to halt the disease progression, but their elucidation and possible use as a therapeutic target represents an important aim of ALS research. Genetic animal models of disease represent useful tools to validate proven hypotheses or to test potential therapies, and the conception of novel hypotheses about ALS causes or the study of pathogenic mechanisms may be advantaged by the use of relatively simple in vivo models recapitulating specific aspects of the disease, thus avoiding the inclusion of too many confounding factors in an experimental setting. Here, we used a neurotoxic model of spinal motoneuron depletion induced by injection of cholera toxin-B saporin in the gastrocnemius muscle to investigate the possible occurrence of compensatory changes in both the muscle and spinal cord. The results showed that, following the lesion, the skeletal muscle became atrophic and displayed electromyographic activity similar to that observed in ALS patients. Moreover, the changes in muscle fiber morphology were different from that observed in ALS models, thus suggesting that some muscular effects of disease may be primary effects instead of being simply caused by denervation. Notably, we found plastic changes in the surviving motoneurons that can produce a functional restoration probably similar to the compensatory changes occurring in disease. These changes could be at least partially driven by glutamatergic signaling, and astrocytes contacting the surviving motoneurons may support this process.
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页数:18
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