Obp56h Modulates Mating Behavior in Drosophila melanogaster

被引:21
作者
Shorter, John R. [1 ,3 ]
Dembeck, Lauren M. [1 ,4 ]
Everett, Logan J. [1 ]
Morozova, Tatiana V. [1 ]
Arya, Gunjan H. [1 ]
Turlapati, Lavanya [1 ]
St Armour, Genevieve E. [1 ]
Schal, Coby [2 ,3 ]
Mackay, Trudy F. C. [1 ]
Anholt, Robert R. H. [1 ]
机构
[1] North Carolina State Univ, Dept Biol Sci, Program Genet, Raleigh, NC 27695 USA
[2] North Carolina State Univ, Dept Entomol & Plant Pathol, Program Genet, Raleigh, NC 27695 USA
[3] Univ North Carolina Chapel Hill, Dept Genet, 120 Mason Farm Rd,5000 D,Genetic Med Bldg,CB 7264, Chapel Hill, NC 27599 USA
[4] Grad Univ, Okinawa Inst Sci & Technol, Format & Regulat Neuronal Connect Res Unit, Onna Son, Okinawa 9040495, Japan
来源
G3-GENES GENOMES GENETICS | 2016年 / 6卷 / 10期
基金
美国国家卫生研究院;
关键词
odorant binding protein; olfaction; cuticular hydrocarbon; pheromone; 5-tricosene; FlyBook; ODORANT-BINDING PROTEINS; PHEROMONE-BINDING; CUTICULAR HYDROCARBONS; CHEMOSENSORY RECEPTORS; NATURAL VARIATION; OLFACTORY SYSTEM; GENE FAMILY; TASTE; EVOLUTION; RECOGNITION;
D O I
10.1534/g3.116.034595
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Social interactions in insects are driven by conspecific chemical signals that are detected via olfactory and gustatory neurons. Odorant binding proteins (Obps) transport volatile odorants to chemosensory receptors, but their effects on behaviors remain poorly characterized. Here, we report that RNAi knockdown of Obp56h gene expression in Drosophila melanogaster enhances mating behavior by reducing courtship latency. The change in mating behavior that results from inhibition of Obp56h expression is accompanied by significant alterations in cuticular hydrocarbon (CHC) composition, including reduction in 5-tricosene (5-T), an inhibitory sex pheromone produced by males that increases copulation latency during courtship. Whole genome RNA sequencing confirms that expression of Obp56h is virtually abolished in Drosophila heads. Inhibition of Obp56h expression also affects expression of other chemoreception genes, including upregulation of lush in both sexes and Obp83ef in females, and reduction in expression of Obp19b and Or19b in males. In addition, several genes associated with lipid metabolism, which underlies the production of cuticular hydrocarbons, show altered transcript abundances. Our data show that modulation of mating behavior through reduction of Obp56h is accompanied by altered cuticular hydrocarbon profiles and implicate 5-T as a possible ligand for Obp56h.
引用
收藏
页码:3335 / 3342
页数:8
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