First molecular characterization of Giardia duodenalis from goats in Malaysia

被引：29

作者：

Lim, Yvonne A. L. ^{[1
,2
]}

Mahdy, Mohammed A. K. ^{[1
]}

Tan, Tiong K. ^{[1
]}

Goh, Xiang T. ^{[1
]}

Jex, Aaron R. ^{[2
]}

Nolan, Matthew J. ^{[2
]}

Sharma, Reuben S. K. ^{[3
]}

Gasser, Robin B. ^{[2
]}

机构：

[1] Univ Malaya, Dept Parasitol, Fac Med, Kuala Lumpur 50603, Malaysia

[2] Univ Melbourne, Dept Vet Sci, Parkville, Vic 3010, Australia

[3] Univ Putra Malaysia, Dept Vet Pathol & Microbiol, Fac Vet Med, Upm Serdang 43400, Selangor Darul, Malaysia

来源：

MOLECULAR AND CELLULAR PROBES | 2013年 / 27卷 / 01期

基金：

澳大利亚研究理事会;

关键词：

Giardia duodenalis; Small subunit of the nuclear ribosomal RNA; (SSU) gene; Goats; Malaysia; CRYPTOSPORIDIUM-PARVUM; ISOMERASE GENE; PREVALENCE; GENOTYPES; SHEEP; EPIDEMIOLOGY; TRANSMISSION; LAMBS; DOGS; IDENTIFICATION;

D O I：

10.1016/j.mcp.2012.08.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study. 310 faecal samples from goats from eight different farms in Malaysia were tested for the presence of Giardia using a PCR-coupled approach. The nested PCR for SSU amplified products of the expected size (similar to 200 bp) from 21 of 310(6.8%) samples. Sixteen of these 21 products could be sequenced successfully and represented six distinct sequence types. Phylogenetic analysis of the SSU sequence data using Bayesian Inference (BI) identified Giardia assemblages A. B and E. The identification of the 'zoonotic' assemblages A and B suggests that Giardia-infected goats represent a possible reservoir for human giardiasis in Malaysia. (c) 2012 Elsevier Ltd. All rights reserved.

引用

页码：28 / 31

页数：4

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