In vitro evaluation of an RGD-functionalized chitosan derivative for enhanced cell adhesion

被引：61

作者：

Hansson, Annasara ^{[1
,2
,3
]}

Hashom, Nour ^{[1
]}

Falson, Francoise ^{[3
]}

Rousselle, Patricia ^{[2
]}

Jordan, Olivier ^{[1
]}

Borchard, Gerrit ^{[1
]}

机构：

[1] Univ Lausanne, Univ Geneva, Sch Pharmaceut Sci, CH-1211 Geneva, Switzerland

[2] Univ Lyon 1, CNRS, SFR BioSci Gerland Lyon Sud, Inst Biol & Chim Proteines,FRE 3310, F-69367 Lyon, France

[3] Univ Lyon 1, Fac Pharm, F-69373 Lyon, France

来源：

CARBOHYDRATE POLYMERS | 2012年 / 90卷 / 04期

关键词：

Wound healing; Human dermal fibroblast; Chitosan; RGD peptide; Cell adhesion; EXTRACELLULAR-MATRIX; SKIN; MODULATION; INTEGRINS; CHITINS;

D O I：

10.1016/j.carbpol.2012.07.020

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Tissue repair is a spontaneous process that is initiated on wounding. However, if this complex mechanism is impaired or not sufficient the use of biomaterials might increase the chance of successful healing. In this view, an RGD-functionalized polymer was developed to promote dermal healing. A water-soluble chitosan derivative, carboxymethyl-trimethylchitosan (CM-TM-chitosan) was synthesized and GRGDS-moieties were grafted to the backbone at a concentration 01 59 nmol/mg polymer to increase cell-biomaterial interaction. Tested in vitro with cultured human dermal fibroblasts, the developed polymer showed good biocompatibility and the initial adhesion was increased by 3-5 times due to the GRGDS-moieties. Moreover, cell spreading was specific to the interaction with GRGDS, giving a 12-fold increase of cells showing a fully spread morphology within 30 min. Overall. CM-TM-chitosan conjugated with GRGDS-peptides may prove useful as a biomaterial in wound healing. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：1494 / 1500

页数：7

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