Multicolor Super-Resolution DNA Imaging for Genetic Analysis

被引：30

作者：

Baday, Murat ^{[3
]}

Cravens, Aaron ^{[4
]}

Hastie, Alex ^{[2
]}

Kim, HyeongJun ^{[4
]}

Kudeki, Deren E.

Kwok, Pui-Yan ^{[1
]}

Xiao, Ming ^{[2
]}

Selvin, Paul R. ^{[3
,4
,5
]}

机构：

[1] Univ Calif San Francisco, Inst Human Genet, San Francisco, CA 94143 USA

[2] BioNano Genom Inc, San Diego, CA 92121 USA

[3] Univ Illinois, Ctr Biophys & Computat Biol, Urbana, IL 61801 USA

[4] Univ Illinois, Dept Phys, Urbana, IL 61801 USA

[5] Univ Illinois, Ctr Phys Living Cell, Urbana, IL 61801 USA

来源：

NANO LETTERS | 2012年 / 12卷 / 07期

基金：

美国国家科学基金会;

关键词：

Super-resolution; optical DNA mapping; genomic variations; copy-number variations; SHRImP; SHREC; DNA; SINGLE-MOLECULE FLUORESCENCE; GENOME SEQUENCE;

D O I：

10.1021/nl302069q

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Many types of cancer and neurodegenerative diseases are caused by abnormalities and variations in the genome. We have designed a high-resolution imaging technique with high throughput and low cost for determining structural variations of genes related to genetic diseases. We initially mapped all seven nicking sites of Nb.BbvCI endonuclease enzyme on lambda DNA. Then we resolved densely labeled patterns of 107 nicking sites on human BAC DNA that is digested by Nb.BsmI and Nb.BbvCI endonuclease enzymes. This high density resulted in several dyes being closer together than the diffraction limit. Overall, detailed DNA nicking sites mapping with 100bp resolution was achieved, which has the potential to reveal information about genetic variance and to facilitate medical diagnosis of several genetic diseases.

引用

页码：3861 / 3866

页数：6

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