Immobilization of α-Amylase by SBA-15

SBA (Santa Barbara Amorphous)-15 mesoporous material was prepared by hydrothermal synthesis and the sample was characterized by X-ray diffraction and scanning electron microscopy. The immobilization of alpha-amylase by SBA-15 was made by physical adsorption method and the optimal conditions of immobilization of alpha-amylase by SBA-15 were obtained as follows: pH = 5.4, alpha-amylase concentration: 2 mg/mL, immobilization time: 27 h, temperature: 30 degrees C. At the same time, it is concluded that the maximum amount of a alpha-amylase immobilized by SBA-15 was 87.5 mg/g. By studying of chemical analysis, scanning electron microscopy, infrared spectroscopy, X-ray diffraction, low temperature N-2 adsorption-desorption analysis characterization, the alpha-amylase was immobilized on the SBA-15 carrier was proved. Catalytic experimental results indicated that (SBA-15)-(alpha-amylase) composite material has the advantages of high pH value resistance and high temperature resistance compared with the free enzyme and has a higher potential applied value.