Pulsed-light imaging for fluorescence guided surgery under normal room lighting

被引：36

作者：

Sexton, Kristian ^{[1
]}

Davis, Scott C. ^{[1
]}

McClatchy, David, III ^{[1
]}

Valdes, Pablo A. ^{[1
,2
,3
]}

Kanick, Stephen C. ^{[1
]}

Paulsen, Keith D. ^{[1
,2
]}

Roberts, David W. ^{[2
,3
]}

Pogue, Brian W. ^{[1
,2
]}

机构：

[1] Dartmouth Coll, Thayer Sch Engn, Hanover, NH 03755 USA

[2] Dartmouth Coll, Geisel Sch Med, Hanover, NH 03755 USA

[3] Dartmouth Hitchcock Med Ctr, Neurosurg Sect, Lebanon, NH 03756 USA

来源：

OPTICS LETTERS | 2013年 / 38卷 / 17期

关键词：

QUANTITATIVE FLUORESCENCE; MALIGNANT GLIOMA; RESECTION; TISSUE; ACID;

D O I：

10.1364/OL.38.003249

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Fluorescence guided surgery (FGS) is an emerging technology that has demonstrated improved surgical outcomes. However, dim lighting conditions required by current FGS systems are disruptive to standard surgical workflow. We present a novel FGS system capable of imaging fluorescence under normal room light by using pulsed excitation and gated acquisition. Images from tissue-simulating phantoms confirm visual detection down to 0.25 mu M of protoporphyrin IX under 125 mu W/cm(2) of ambient light, more than an order of magnitude lower than that measured with the Zeiss Pentero in the dark. Resection of orthotopic brain tumors in mice also suggests that the pulsed-light system provides superior sensitivity in vivo. (C) 2013 Optical Society of America

引用

页码：3249 / 3252

页数：4

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