Genetic stability of regenerated plants via indirect somatic embryogenesis and indirect shoot regeneration of Carum copticum L.

被引:40
作者
Niazian, Mohsen [1 ]
Noori, Seyed Ahmad Sadat [1 ]
Galuszka, Petr [2 ]
Tohidfar, Masoud [3 ]
Mortazavian, Seyed Mohammad Mandi [1 ]
机构
[1] Univ Tehran, Coll Aburaihan, Dept Agron & Plant Breeding Sci, Tehran, Iran
[2] Palacky Univ, Ctr Reg Hana Biotechnol & Agr Res, Dept Mol Biol, Olomouc, Czech Republic
[3] Shahid Beheshti Univ, Fac New Technol & Energy Engn, Dept Biotechnol, GC, Tehran, Iran
关键词
Ajowan; Embryogenesis; Explant age; Flow cytometry; Hypocotyl; EXPLANTS; CULTURE; CALLUS; SYSTEM; SEED;
D O I
10.1016/j.indcrop.2016.12.044
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Ajowan (Carum copticum L.) is an important and endangered industrial medicinal plant that growing in some parts of Iran. Two efficient protocols, without somaclonal variation induction, were developed for indirect somatic embryogenesis and indirect shoot regeneration of three Iranian ecotypes of ajowan. In the first experiment, higher concentration of auxin than cytokinin (1, 1.5 and 2 mg/L of 2,4-dichlorophenoxyacetic acid along with 0.25, 0.5 and 0.75 mg/L kinetin) was used for callus induction in 5, 10 and 15 days old hypocotyl explants. In the second experiment, higher concentration of cytokinin than auxin (1 mg/L of kinetin along with 0.5 mg/L of 2,4-dichlorophenoxyacetic acid) was used for callus induction in 15-d old hypocotyl explants. The higher frequency of somatic embryos was achieved from 15 days old hypocotyl explants of Ghoom ecotype with 28.33 embryos when induced calli from MS medium supplemented with 1.5 mg/L 2,4-dichlorophenoxyacetic acid in combination with 0.5 mg/L kinetin transferred to free plant growth regulator MS medium. Momentary removing of 2,4-dichlorophenoxyacetic acid was successful for somatic embryogenesis in Iranian ecotypes of ajowan that it significantly reduce the time of the culture and thus reduce the risk of somaclonal variation. Maximum number of initiated shoots per explant was related to Shiraz ecotype with average 18.33 shoots per callus in MS medium supplemented with 1.5 mg/L of specify type of cytokinin (3-methoxy [-6-benzylamino-9-tetrahydropyran-2-yl] purine) plus 0.25 mg/L naphathalene acetic acid. The survival rate of rooted plantlets was 60.86% and 58.33% for indirect somatic embryogenesis and indirect shoot regeneration derived plants, respectively. The genetic stability of regenerated plants via indirect somatic embryogenesis and indirect shoot regeneration was proved through flow cytometry analysis. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:330 / 337
页数:8
相关论文
共 50 条
[21]   Copper nanoparticles elevate regeneration capacity of (Ocimum basilicum L.) plant via somatic embryogenesis [J].
Ibrahim, Ahmed S. ;
Fahmy, Ashraf H. ;
Ahmed, Shreen S. .
PLANT CELL TISSUE AND ORGAN CULTURE, 2019, 136 (01) :41-50
[22]   PLANTLET REGENERATION THROUGH SOMATIC EMBRYOGENESIS IN SCHISANDRA CHINENSIS (TURCZ.) BAILL. AND ANALYSIS OF GENETIC STABILITY OF REGENERATED PLANTS BY SRAP MARKERS [J].
Sun, Dan ;
Li, Qian ;
Li, Hongbo ;
Ai, Jun ;
Qin, Hong Yan ;
Piao, Zhong Yun .
BANGLADESH JOURNAL OF BOTANY, 2015, 44 (05) :881-888
[23]   In vitro regeneration of Phaseolus vulgaris L. via direct and indirect organogenesis [J].
Yu, Yan ;
Liu, Dajun ;
Liu, Chang ;
Yan, Zhishan ;
Yang, Xiaoxu ;
Feng, Guojun .
PLANT BIOTECHNOLOGY REPORTS, 2021, 15 (03) :279-288
[24]   Regeneration of miniature potted rose (Rosa hybrida L.) via somatic embryogenesis [J].
Zakizadeh, H. ;
Debener, T. ;
Sriskandarajah, S. ;
Frello, S. ;
Serek, M. .
EUROPEAN JOURNAL OF HORTICULTURAL SCIENCE, 2008, 73 (03) :111-117
[25]   Regeneration of different cultivars of common bean (Phaseolus vulgaris L.) via indirect organogenesis [J].
Arellano, Jesus ;
Fuentes, Sara Isabel ;
Castillo-Espana, Patricia ;
Hernandez, Georgina .
PLANT CELL TISSUE AND ORGAN CULTURE, 2009, 96 (01) :11-18
[26]   Plantlet regeneration through indirect shoot organogenesis and somatic embryogenesis in Justicia gendarussa Burm. f., a medicinal plant [J].
Bhagya, N. ;
Chandrashekar, K. R. ;
Karun, Anitha ;
Bhavyashree, U. .
JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY, 2013, 22 (04) :474-482
[27]   Autotetraploid plant regeneration by indirect somatic embryogenesis from leaf mesophyll protoplasts of diploid Gentiana decumbens L.f. [J].
Tomiczak, Karolina ;
Mikua, Anna ;
Sliwinska, Elwira ;
Rybczynski, Jan J. .
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, 2015, 51 (03) :350-359
[28]   Regeneration of soybean (Glycine max L. Merrill) through direct somatic embryogenesis from the immature embryonic shoot tip [J].
Loganathan, Murugan ;
Maruthasalam, Subbiyan ;
Shiu, Ling Yin ;
Lien, Wei Ching ;
Hsu, Wen Hwei ;
Lee, Pei Fang ;
Yu, Chih Wen ;
Lin, Chin Ho .
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, 2010, 46 (03) :265-273
[29]   Plant regeneration via somatic embryogenesis in diploid cultivated cotton (Gossypium arboreum L.) [J].
Ke, Liping ;
Jiang, Qimeng ;
Wang, Rongjia ;
Yu, Dongliang ;
Sun, Yuqiang .
PLANT CELL TISSUE AND ORGAN CULTURE, 2022, 148 (01) :177-188
[30]   Somatic embryogenesis of muskmelon (Cucumis melo L.) and genetic stability assessment of regenerants using flow cytometry and ISSR markers [J].
Raji, Mohammad Reza ;
Lotfi, Mahmoud ;
Tohidfar, Masoud ;
Zahedi, Bahman ;
Carra, Angela ;
Abbate, Loredana ;
Carimi, Francesco .
PROTOPLASMA, 2018, 255 (03) :873-883