Free radical scavenging and antioxidant enzyme fortifying activities of extracts from Smilax china root

被引：60

作者：

Lee, SE ^{[1
]}

Ju, EM ^{[1
]}

Kim, JH ^{[1
]}

机构：

[1] Kyung Hee Univ, Coll Dent, Dept Oral Biochem, Seoul 130701, South Korea

来源：

EXPERIMENTAL AND MOLECULAR MEDICINE | 2001年 / 33卷 / 04期

关键词：

antioxidant activity; antioxidant enzymes; lipid peroxidation; radical scavenging; Smilax china root;

D O I：

10.1038/emm.2001.43

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The extract from Smilax china root has been used as medicinal remedy and reported to retain antimicrobial and antimutagenic acitivities. In this study, a possible presence of antioxidant activity of Smilax china root extract was investigated. Methanol extract (Me) revealed the presence of high 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity (IC50 7.4 mug/ml) and protective property of cell's viability. Further fractionation with various solvent extraction and assay showed high levels of DPPH free radical scavenging activity in the ethyl acetate, butanol and water extracted fractions. In addition, V79-4 cells treated with Me of Smilax china root induced an increase of superoxide dismutase, catalase and glutathione peroxidase activities in a dose-dependent manner between 4-100 mug/ml. These results suggest that the medicinal component of the root of Smilax china extracts also contains antioxidant activity.

引用

页码：263 / 268

页数：6

共 34 条

[1] SUPEROXIDE DISMUTASE - IMPROVED ASSAYS AND AN ASSAY APPLICABLE TO ACRYLAMIDE GELS [J].

BEAUCHAM.C ;

FRIDOVIC.I .

ANALYTICAL BIOCHEMISTRY, 1971, 44 (01) :276-&

[2] Anti-oxidant effect of Withania somnifera glycowithanolides in chronic footshock stress-induced perturbations of oxidative free radical scavenging enzymes and lipid peroxidation in rat frontal cortex and striatum [J].

Bhattacharya, A ;

Ghosal, S ;

Bhattacharya, SK .

JOURNAL OF ETHNOPHARMACOLOGY, 2001, 74 (01) :1-6

[3] FREE-RADICALS IN BIOLOGICAL-SYSTEMS - A REVIEW ORIENTATED TO INFLAMMATORY PROCESSES [J].

BLAKE, DR ;

ALLEN, RE ;

LUNEC, J .

BRITISH MEDICAL BULLETIN, 1987, 43 (02) :371-385

[4]

Blos M.S., 1958, NATURE, V181, P1199

[5]

BOREK C, 1991, FREE RADICAL RES COM, V12-3, P745

[6]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[7] Luteolin-rich artichoke extract protects low density lipoprotein from oxidation in vitro [J].

Brown, JE ;

Rice-Evans, CA .

FREE RADICAL RESEARCH, 1998, 29 (03) :247-255

[8] Antioxidant and prooxidant behavior of flavonoids: Structure-activity relationships [J].

Cao, GH ;

Sofic, E ;

Prior, RL .

FREE RADICAL BIOLOGY AND MEDICINE, 1997, 22 (05) :749-760

[9] Inhibition of peroxynitrite dependent DNA base modification and tyrosine nitration by the extra virgin olive oil-derived antioxidant hydroxytyrosol [J].

Deiana, M ;

Aruoma, OI ;

Bianchi, MDP ;

Spencer, JPE ;

Kaur, H ;

Halliwell, B ;

Aeschbach, R ;

Banni, S ;

Dessi, MA ;

Corongiu, FP .

FREE RADICAL BIOLOGY AND MEDICINE, 1999, 26 (5-6) :762-769

[10] Antioxidant activity of mung bean hulls [J].

Duh, PD ;

Yen, WJ ;

Du, PC ;

Yen, GC .

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1997, 74 (09) :1059-1063

← 1 2 3 4 →