Agrobacterium-mediated transformation of the haploid liverwort Marchantia polymorpha L., an emerging model for plant biology

被引：265

作者：

Ishizaki, Kimitsune ^{[1
]}

Chiyoda, Shota ^{[1
]}

Yamato, Katsuyuki T. ^{[1
]}

Kohchi, Takayuki ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Biostudies, Kyoto 6068502, Japan

来源：

PLANT AND CELL PHYSIOLOGY | 2008年 / 49卷 / 07期

基金：

日本学术振兴会;

关键词：

Agrobacterium-mediated transformation; bryophyte; hepaticae; liverwort; Marchantia polymorpha; T-DNA;

D O I：

10.1093/pcp/pcn085

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Agrobacterium-mediated transformation has not been practical in pteridophytes, bryophytes and algae to date, although it is commonly used in model plants including Arabidopsis and rice. Here we present a rapid Agrobacterium-mediated transformation system for the haploid liverwort Marchantia polymorpha L. using immature thalli developed from spores. Hundreds of hygromycin-resistant plants per sporangium were obtained by co-cultivation of immature thalli with Agrobacterium carrying the binary vector that contains a reporter, the beta-glucuronidase (GUS) gene with an intron, and a selection marker, the hygromycin phosphotransferase (hpt) gene. In this system, individual gemmae, which arise asexually from single initial cells, were analyzed as isogenic transformants. GUS activity staining showed that all hygromycin-resistant plants examined expressed the GUS transgene in planta. DNA analyses verified random integration of 1-5 copies of the intact T-DNA between the right and the left borders into the M. polymorpha genome. The efficient and rapid Agrobacterium-mediated transformation of M. polymorpha should provide molecular techniques to facilitate comparative genomics, taking advantage of this unique model plant that retains many features of the common ancestor of land plants.

引用

页码：1084 / 1091

页数：8

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