Effects of organic solvents on activity and conformation of recombinant Candida antarctica lipase A produced by Pichia pastoris

被引：34
作者：
Yang, Chunfang [2 ]
Wang, Fanghua [2 ]
Lan, Dongming [3 ]
Whiteley, Chris [4 ]
Yang, Bo [1 ]
Wang, Yonghua [2 ]
机构：
[1] S China Univ Technol, Dept Biotechnol, Sch Biosci & Bioengn, Guangzhou 510006, Guangdong, Peoples R China
[2] S China Univ Technol, Coll Light Ind & Food Sci, Guangzhou 510640, Guangdong, Peoples R China
[3] S China Univ Technol, Sch Chem & Chem Engn, Guangzhou 510640, Guangdong, Peoples R China
[4] Rhodes Univ, Dept Biochem Microbiol & Biotechnol, ZA-6140 Grahamstown, South Africa
来源：
PROCESS BIOCHEMISTRY | 2012年 / 47卷 / 03期
关键词：
Candida antarctica lipase A; Organic solvents; Catalytic activity; Secondary structure; Fourier transform-infrared spectroscopy; EXPRESSION; ESTERIFICATION; PURIFICATION; SHOWS;
D O I：
10.1016/j.procbio.2011.11.017
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effect of various organic solvents on the hydrolytic and esterification activities of lipase A from Candida antarctica (CALA) was investigated. Compared to the control group, the esterification and hydrolytic activities of CALA both increased with treatment of acetonitrile or acetone. However, the catalytic activity decreased after treatment with other organic solvents, especially with ethanol or ethyl acetate. Moreover, with treatment by the same organic solvents, the residual esterification activity of CALA was much higher than the hydrolytic activity for the olive oil. Results of Fourier transform-infrared spectroscopy analysis implied that the catalytic activity variance was mainly attributed to the secondary structure changes of CALA. The acetonitrile treatment could also increase the thermal and pH stability of CALA. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：533 / 537
页数：5
相关论文
共 22 条
[1]   Secondary structure analysis of HIV-1-gp41 in solution and adsorbed to aluminum hydroxide by Fourier transform infrared spectroscopy [J].
Agopian, Audrey ;
Ronzon, Frederic ;
Sauzeat, Elisabeth ;
Sodoyer, Regis ;
El Habib, Raphaelle ;
Buchet, Rene ;
Chevalier, Michel .
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2007, 1774 (03) :351-358
[2]   THE FUTURE-IMPACT OF INDUSTRIAL LIPASES [J].
BJORKLING, F ;
GODTFREDSEN, SE ;
KIRK, O .
TRENDS IN BIOTECHNOLOGY, 1991, 9 (10) :360-363
[3]   Lipase-catalyzed enantioselective esterification of ibuprofen in organic solvents under controlled water activity [J].
Ducret, A ;
Trani, M ;
Lortie, R .
ENZYME AND MICROBIAL TECHNOLOGY, 1998, 22 (04) :212-216
[4]   X-ray structure of Candida antarctica lipase a shows A novel lid structure and a likely mode of interfacial activation [J].
Ericsson, Daniel J. ;
Kasrayan, Alex ;
Johanssonl, Patrik ;
Bergfors, Terese ;
Sandstrom, Anders G. ;
Backvall, Jan-E. ;
Mowbray, Sherry L. .
JOURNAL OF MOLECULAR BIOLOGY, 2008, 376 (01) :109-119
[5]   A newly isolated organic solvent tolerant Staphylococcus saprophyticus M36 produced organic solvent-stable lipase [J].
Fang, Yaowei ;
Lu, Zhaoxin ;
Lv, Fengxia ;
Bie, Xiaomei ;
Liu, Shu ;
Ding, Zhongyang ;
Xu, Weifeng .
CURRENT MICROBIOLOGY, 2006, 53 (06) :510-515
[6]   Expression and characterization of a novel enantioselective lipase from Acinetobacter species SY-01 [J].
Han, SJ ;
Back, JH ;
Yoon, MY ;
Shin, PK ;
Cheong, CS ;
Sung, MH ;
Hong, SP ;
Chung, IY ;
Han, YS .
BIOCHIMIE, 2003, 85 (05) :501-510
[7]   Immobilised Burkholderia cepacia lipase in dry organic solvents and ionic liquids: A comparison [J].
Hara, Piia ;
Hanefeld, Ulf ;
Kanerv, Liisa T. .
GREEN CHEMISTRY, 2009, 11 (02) :250-256
[8]   FTIR spectroscopic characterization of protein structure in aqueous and non-aqueous media [J].
Haris, PI ;
Severcan, F .
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 1999, 7 (1-4) :207-221
[9]   Effects of organic solvents on activity and stability of lipases produced by thermotolerant fungi in solid-state fermentation [J].
Hernandez-Rodriguez, Blanca ;
Cordova, Jesus ;
Barzana, Eduardo ;
Favela Torres, Ernesto .
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2009, 61 (3-4) :136-142
[10]  
KILBANOV AM, 1986, CHEMTECH, V16, P354
123