Effect of Autologous Adipose-Derived Stem Cells in Renal Cold Ischemia and Reperfusion Injury

Objective. The aim of this study was to investigate the effect of autologous adiposederived stem cells (ADSCs) on renal cold ischemia and reperfusion (I/R) injury via intravenous infusion on rats. Methods. A renal cold I/R injury rat model was established. Rats were equally randomized into Sham group, Cold I/R group (cold I/R plus culture medium only), and ADSC-treated group (cold I/R plus immediate intrarenal administration of 2 x 10(6) autologous ADSCs, followed by intravenous autologous ADSCs 6 hours after reperfusion). All rats were killed 24 hours after the I/R procedure. Results. Serum creatinine levels were significantly reduced in the ADS C-treated group compared with the Cold I/R group (P <.01). The renal tissue in the ADSC-treated group had well conserved renal architecture compared with the Cold I/R group. The mRNA expression of tumor necrosis factor alpha was significantly lower and Bcl-2 was higher in the ADSC-treated group than in the Cold I/R group (P <.05). Conclusions. Autologous ADSC infusions ameliorated renal damage undergoing cold I/R injury and improved the renal function, partly through inhibiting inflammatory reactions and reducing apoptosis.