Identification of brassinosteroid responsive genes in Arabidopsis by cDNA array

被引：10
作者：
Hu, YX
Wang, ZK
Wang, YH
Bao, F
Li, N
Peng, ZH
Li, JY [1 ]
机构：
[1] Chinese Acad Sci, Inst Genet, Beijing 100101, Peoples R China
[2] Chinese Acad Forestry, Inst Forest, Beijing 100091, Peoples R China
[3] Hong Kong Univ Sci & Technol, Dept Biol, Kowloon, Hong Kong, Peoples R China
来源：
SCIENCE IN CHINA SERIES C-LIFE SCIENCES | 2001年 / 44卷 / 06期
关键词：
cDNA array; brassinosteroid responsive gene (BRR); Arabidopsis thaliana;
D O I：
10.1007/BF02879358
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We have systematically monitored brassinosteroid (BR) responsive genes in a BR-deficient mutant det2 suspension culture of Arabidopsis by using a cDNA array approach. Among 13000 cDNA clones arrayed on filters, 53 BR responsive clones were identified and designated BRR1-BRR53. Sequence analysis of 43 clones showed that 19 clones are novel genes, 3 clones are genes involved in the control of cell division, 4 clones are genes related to plant stress responses, 4 clones are transcriptional factor or signal transduction component genes, and 3 clones are genes involved in RNA splicing or structure forming. In addition, we also found that BR regulated the transcription of genes related to many physiological processes, such as photoreaction, ion transportation and some metabolic processes. These findings present molecular evidence that BR plays an essential role in plant growth and development.
引用
收藏
页码：637 / 643
页数：7
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