Antibiotic resilience in Xanthomonas axonopodis pv. punicae causing bacterial blight of pomegranate

被引:5
|
作者
Krishna, Priyanka [1 ]
Kumar, M. K. Prasanna [1 ]
Channappa, Manjunatha [2 ]
Devanna, Pramesh [3 ]
Singh, Kartar [4 ]
Eeregowda, Puneeth M. [1 ]
Mahesh, H. B. [1 ]
Chandrashekar, B. S. [1 ]
Babu, Venkatesh [5 ]
Desai, Radhika U. [1 ]
Banakar, Sahana N. [1 ]
机构
[1] Univ Agr Sci UAS, Dept Plant Pathol, Bengaluru 560065, India
[2] ICAR Indian Agr Res Inst, Reg Stn, Wellington 643231, India
[3] UAS, Agr Res Stn, Raichur 584104, India
[4] ICAR Natl Bur Plant Genet Resources, Reg Stn, Jodhpur 342005, Rajasthan, India
[5] Univ Madras, Ctr Adv Studies Bot, Guindy Campus, Chennai 600005, Tamil Nadu, India
来源
CURRENT SCIENCE | 2020年 / 119卷 / 09期
关键词
Antibiotics; bacterial blight; copper compounds; disc diffusion; pomegranate; resistant genes; COPPER RESISTANCE GENES; STREPTOMYCIN RESISTANCE; PSEUDOMONAS-SYRINGAE; SUBSP CITRI; SENSITIVITY; CAMPESTRIS; SEQUENCE;
D O I
10.18520/cs/v119/i9/1564-1569
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Bacterial blight caused by Xanthomonas axonopodis pv. punicae (Xap) is one of the economically important diseases of pomegranate in India. Under field conditions, the disease is being managed using antibiotics and copper-based compounds but with limited success due to their poor bio-efficacy. The reduced efficacy of antibiotics and copper compounds against field populations of Xap might be due to the development of bactericide resistance through acquired genes. In the present study, ten bacterial blight-infected pomegranate samples were collected from different geographic locations of Karnataka, India, and causal agent Xap was isolated and identified through 16S rRNA sequencing. Streptomycin resistance genes such as rpsL, strA, strB and copper resistance genes copL, copB were detected using gene-specific primers in PCR. All ten isolates were positive for streptomycin resistance genes whereas copper resistance gene copB was absent in three isolates (Xap1, Xap4, Xap6) while copL was absent in Xap4 and Xap6 isolates. Further, in vitro experiments using different concentrations of streptomycin on culture media showed lowest growth inhibition up to 1500 mu g/ml concentration, supporting the molecular evidence of antibiotic resistance. The present study provides preliminary information on the presence of antibiotic resistance genes in the field populations of Xap.
引用
收藏
页码:1564 / 1569
页数:7
相关论文
共 50 条
  • [1] Detection of Xanthomonas axonopodis pv. punicae causing bacterial blight on pomegranate in South Africa
    Y. Petersen
    E. L. Mansvelt
    E. Venter
    W. E. Langenhoven
    Australasian Plant Pathology, 2010, 39 : 544 - 546
  • [2] Detection of Xanthomonas axonopodis pv. punicae causing bacterial blight on pomegranate in South Africa
    Petersen, Y.
    Mansvelt, E. L.
    Venter, E.
    Langenhoven, W. E.
    AUSTRALASIAN PLANT PATHOLOGY, 2010, 39 (06) : 544 - 546
  • [3] Epidemiology and Management of Bacterial Blight of Pomegranate Caused by Xanthomonas axonopodis pv. punicae
    Kumar, R.
    Jahagirdar, M. R. Shamarao
    Yenjerappa, S. T.
    Patil, H. B.
    I INTERNATIONAL SYMPOSIUM ON POMEGRANATE AND MINOR MEDITERRANEAN FRUITS, 2009, 818 : 291 - 295
  • [4] First Report of Bacterial Blight of Pomegranate Caused by Xanthomonas axonopodis pv. punicae in Turkey
    Icoz, S. M.
    Polat, I.
    Sulu, G.
    Yilmaz, M.
    Unlu, A.
    Soylu, S.
    Bozkurt, I. A.
    Baysal, O.
    PLANT DISEASE, 2014, 98 (10) : 1427 - 1427
  • [5] Lytic bacteriophages in preventing the bacterial blight of pomegranate caused by Xanthomonas axonopodis pv. punicae
    Karn M.
    Sharma S.K.
    Handa A.
    Sharma A.
    Sharma S.
    Sharma U.
    Vegetos, 2023, 36 (2): : 643 - 650
  • [6] POMEGRANATE BACTERIAL BLIGHT: SYMPTOMATOLOGY AND RAPID INOCULATION TECHNIQUE FOR XANTHOMONAS AXONOPODIS pv. PUNICAE
    Sharma, J.
    Sharma, K. K.
    Kumar, A.
    Mondal, K. K.
    Thalor, S.
    Maity, A.
    Gharate, R.
    Chinchure, S.
    Jadhav, V. T.
    JOURNAL OF PLANT PATHOLOGY, 2017, 99 (01) : 109 - 119
  • [7] In vitro Prospects of Botanicals, Bioagents and Micronutrients in the Inhibition of Xanthomonas axonopodis pv. Punicae Causing Bacterial Blight of Pomegranate
    Basamma
    Benagi, V. I.
    Nargund, V. B.
    Nirmalnath, Jones
    JOURNAL OF PURE AND APPLIED MICROBIOLOGY, 2014, 8 (01): : 727 - 732
  • [8] Polyphasic phenotypic and genetic analysis reveals clonal nature of Xanthomonas axonopodis pv. punicae causing pomegranate bacterial blight
    Kumar, A.
    Sharma, J.
    Munjal, V.
    Sakthivel, K.
    Thalor, S. K.
    Mondal, K. K.
    Chinchure, S.
    Gharate, R.
    PLANT PATHOLOGY, 2020, 69 (02) : 347 - 359
  • [9] Present Status of Pomegranate Bacterial Blight Caused by Xanthomonas axonopodis pv. punicae and Its Management
    Sharma, K. K.
    Jadhav, V. T.
    Sharma, J.
    II INTERNATIONAL SYMPOSIUM ON POMEGRANATE AND MINOR - INCLUDING MEDITERRANEAN - FRUITS: ISPMMF2009, 2011, 890 : 513 - 522
  • [10] Identification and validation of SSR markers for Xanthomonas axonopodis pv. punicae an incitant of bacterial blight of pomegranate
    Prakash G. Patil
    Jyotsana Sharma
    Manjunatha Nanjundappa
    N. V. Singh
    Abhishek Bohra
    Raghavendra Gunnaiah
    Shivani M. Jamma
    Jeer Vinayaka
    Vipul R. Sangnure
    R. A. Marathe
    3 Biotech, 2022, 12