Crystallization and Preliminary X-Ray Diffraction Analysis of a Novel Mannose-Binding Lectin with Antiretroviral Properties from Polygonatum cyrtonema Hua

被引：7

作者：

Ding, Jing-Jin ^{[1
,2
]}

Bao, Jin-ku ^{[3
]}

Zhu, De-Yu ^{[1
,2
]}

Zhang, Ying ^{[1
]}

Wang, Da-Cheng ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China

[2] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China

[3] Sichuan Univ, Coll Life Sci, Chengdu 610064, Sichuan, Peoples R China

来源：

PROTEIN AND PEPTIDE LETTERS | 2008年 / 15卷 / 04期

关键词：

PCL; monocot mannose-binding lectin; crystallization; preliminary X-ray diffraction analysis; Polygonatum cyrtonema Hua;

D O I：

10.2174/092986608784246551

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel antiretroviral protein Polygonatum cyrtonema lectin (PCL) belonging to the monocot mannose-binding lectin (MMBL) superfamily has been crystallized using hanging-drop vapor-diffusion method. The crystals diffract to 2.0 angstrom resolution and belong to space group P2(1), with unit-cell parameters of a=39.308 angstrom, b=48.317 angstrom, c=112.221 angstrom, and beta=90.12 degrees. Preliminary analysis indicates that the asymmetric unit contains four PCL molecules with a solvent content of about 45%. A set of X-ray data has been collected for the crystal structure determination.

引用

页码：411 / 414

页数：4

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