Exposure to cigarette smoke downregulates β2-adrenergic receptor expression and upregulates inflammation in alveolar macrophages

被引:17
作者
Wang, Wei [1 ]
Li, Xiaoguang [1 ]
Xu, Jie [1 ]
机构
[1] Peking Univ, Hosp 3, Dept Infect Dis, Beijing 100191, Peoples R China
关键词
beta 2-Adrenergic receptor; alveolar macrophages; cigarette smoke; inflammation; FACTOR-KAPPA-B; CYTOKINE PRODUCTION; LUNG INFLAMMATION; ACTIVATION; SPUTUM; COPD; TRANSCRIPTION;
D O I
10.3109/08958378.2015.1075628
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
Cigarette smoke-triggered inflammation is important in the pathophysiology of chronic obstructive pulmonary disease (COPD). beta(2)-Adrenergic receptor (beta(2)-AR) is abundantly expressed on inflammatory cells, which is associated with inflammation regulation. To observe alterations in inflammation, pathological changes in lung tissues, and detect changes in beta(2)-AR expression, rats were exposed for 4 months to cigarette smoke. Pathological changes were observed in lung tissue sections. The levels of inflammatory mediators tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta in bronchoalveolar lavage fluid (BALF), and lung tissues were measured using enzyme-linked immunosorbent assay (ELISA). Nuclear factor (NF)-kappa B activity was detected by electrophoretic mobility shift assay (EMSA). Exposure to this regimen of cigarette smoke induced peribronchial and perivascular lymphocytic aggregates and parenchymal accumulation of macrophages in rats. EMSA demonstrated that smoke exposure enhanced NF-kappa B activation in rats' alveolar macrophages (AMs). Compared with the control group, smoke exposure induced a notable increase in TNF-alpha and IL-1 beta in BALF, lung tissues, and a decrease of beta(2)-AR expression of AMs. The expression of beta(2)-AR from AMs was inversely correlated with TNF-alpha and IL-1 beta levels of BALF. These data demonstrated that chronic smoke-triggered lung inflammation was accompanied by down-regulation of beta(2)-AR in rat lungs' AMs.
引用
收藏
页码:488 / 494
页数:7
相关论文
共 25 条
[1]   MR imaging and targeting of a specific alveolar macrophage subpopulation in LPS-induced COPD animal model using antibody-conjugated magnetic nanoparticles [J].
Al Faraj, Achraf ;
Shaik, Asma Sultana ;
Afzal, Sibtain ;
Al Sayed, Baraa ;
Halwani, Rabih .
INTERNATIONAL JOURNAL OF NANOMEDICINE, 2014, 9 :1491-1503
[2]  
Calveriey PMA, 2003, CHRONIC OBSTRUCTIVE, P131
[3]   Tumor necrosis factor-α drives 70% of cigarette smoke-induced emphysema in the mouse [J].
Churg, A ;
Wang, RD ;
Tai, H ;
Wang, XS ;
Xie, CS ;
Wright, JL .
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2004, 170 (05) :492-498
[4]   CXCR3 and CCR5 chemokines in induced sputum from patients with COPD [J].
Costa, Claudia ;
Rufino, Rogerio ;
Traves, Suzanne L. ;
Lapa e Silva, Jose Roberto ;
Barnes, Peter J. ;
Donnelly, Louise E. .
CHEST, 2008, 133 (01) :26-33
[5]   Increased expression of nuclear factor-κB in bronchial biopsies from smokers and patients with COPD [J].
Di Stefano, A ;
Caramori, G ;
Oates, T ;
Capelli, A ;
Lusuardi, M ;
Gnemmi, I ;
Ioli, F ;
Chung, KF ;
Donner, CF ;
Barnes, PJ ;
Adcock, IM .
EUROPEAN RESPIRATORY JOURNAL, 2002, 20 (03) :556-563
[6]   COPD: Beyond the respiratory system [J].
Escamilla, Roger .
PRESSE MEDICALE, 2014, 43 (12) :1381-1386
[7]   β-Adrenergic agonists exert their "anti-inflammatory" effects in monocytic cells through the IκB/NF-κB pathway [J].
Farmer, P ;
Pugin, J .
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2000, 279 (04) :L675-L682
[8]   Endogenous β-adrenergic receptors inhibit lipopolysaccharide-induced pulmonary cytokine release and coagulation [J].
Giebelen, Ida A. J. ;
Leendertse, Masia ;
Dessing, Mark C. ;
Meijers, Joost C. M. ;
Levi, Marcel ;
Draing, Christian ;
von Aulock, Sonja ;
van der Poll, Tom .
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2008, 39 (03) :373-379
[9]   Effects of one month treatment with propranolol and metoprolol on the relaxant and contractile function of isolated trachea from rats exposed to cigarette smoke for four months [J].
Guo, Yang ;
Zhang, Yuan ;
Shen, Ning ;
Zhou, Yujiao ;
Zhang, Youyi ;
Wupuer, Halmurat ;
He, Bei .
INHALATION TOXICOLOGY, 2014, 26 (05) :271-277
[10]   Clinical characteristics and airway inflammation profile of COPD persistent sputum producers [J].
Khurana, S. ;
Ravi, A. ;
Sutula, J. ;
Milone, R. ;
Williamson, R. ;
Plumb, J. ;
Vestbo, J. ;
Singh, D. .
RESPIRATORY MEDICINE, 2014, 108 (12) :1761-1770