Mobilization of Pack-CACTA transposons in Arabidopsis suggests the mechanism of gene shuffling

被引：22

作者：

Catoni, Marco ^{[1
,2
]}

Jonesman, Thomas ^{[1
]}

Cerruti, Elisa ^{[1
]}

Paszkowski, Jerzy ^{[1
]}

机构：

[1] Univ Cambridge, Sainsbury Lab, Cambridge CB2 1LR, England

[2] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England

来源：

NUCLEIC ACIDS RESEARCH | 2019年 / 47卷 / 03期

基金：

欧洲研究理事会;

关键词：

DNA METHYLATION; GAP REPAIR; ELEMENT; EXCISION; DROSOPHILA; THALIANA; RETROTRANSPOSITION; MUTATION; MULES;

D O I：

10.1093/nar/gky1196

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pack-TYPE transposons are a unique class of potentially mobile non-autonomous elements that can capture, merge and relocate fragments of chromosomal DNA. It has been postulated that their activity accelerates the evolution of host genes. However, this important presumption is based only on the sequences of currently inactive Pack-TYPE transposons and the acquisition of chromosomal DNA has not been recorded in real time. Analysing the DNA copy number variation in hypomethylated Arabidopsis lines, we have now for the first time witnessed the mobilization of novel Pack-TYPE elements related to the CACTA transposon family, over several plant generations. Remarkably, these elements can insert into genes as closely spaced direct repeats and they frequently undergo incomplete excisions, resulting in the deletion of one of the end sequences. These properties suggest a mechanism of efficient acquisition of genic DNA residing between neighbouring Pack-TYPE transposons and its subsequent mobilization. Our work documents crucial steps in the formation of in vivo novel Pack-TYPE transposons, and thus the possible mechanism of gene shuffling mediated by this type of mobile element.

引用

页码：1311 / 1320

页数：10

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