Haptoglobin and CCR2 receptor expression in ovarian cancer cells that were exposed to ascitic fluid: Exploring a new role of haptoglobin in the tumoral microenvironment

被引:16
作者
Garibay-Cerdenares, O. L. [1 ]
Hernandez-Ramirez, V. I. [1 ]
Osorio-Trujillo, J. C. [1 ]
Gallardo-Rincon, D. [2 ]
Talamas-Rohana, P. [1 ]
机构
[1] IPN, CINVESTAV, Dept Infect & Patogenesis Mol, Mexico City 07738, DF, Mexico
[2] Inst Nacl Cancerol, Dept Oncol Med, Mexico City, DF, Mexico
基金
芬兰科学院;
关键词
actin cytoskeleton; ascitic fluid; CCR2; cell migration; haptoglobin; ovarian cancer; RS102895; MESENCHYMAL TRANSITION; SERUM HAPTOGLOBIN; IDENTIFICATION; BIOMARKER; PROTEINS; GENE; POLYMORPHISM; TRANSFERRIN; CARCINOMA; MIGRATION;
D O I
10.1080/19336918.2015.1035504
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Haptoglobin (Hp) is an acute-phase protein that is produced by the liver to capture the iron that is present in the blood circulation, thus avoiding its accumulation in the blood. Moreover, Hp has been detected in a wide variety of tissues, in which it performs various functions. In addition, this protein is considered a potential biomarker in many diseases, such as cancer, including ovarian carcinoma; however, its participation in the cancerous processes has not yet been determined. The objective of this work was to demonstrate the expression of Hp and its receptor CCR2 in the ovarian cancer cells and its possible involvement in the process of cell migration through changes in the rearrangement of the actin cytoskeleton using western blot and wound-healing assays and confirming by confocal microscopy. Ovarian cancer cells express both Hp and its receptor CCR2 but only after exposure to ascitic fluid, inducing moderated cell migration. However, when the cells are exposed to exogenous Hp, the expression of CCR2 is induced together with drastic changes in the actin cytoskeleton rearrangement. At the same time, Hp induced cell migration in a much more efficient manner than did ascitic fluid. These effects were blocked when the CCR2 synthetic antagonist RS102895 was used to pretreat the cells. These results suggest that Hp-induced changes in the cell morphology, actin cytoskeleton structure, and migration ability of tumor cells, is possibly preparing these cells for the potential induction of the metastatic phenotype.
引用
收藏
页码:394 / 405
页数:12
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