DNA-detection of leaf curl pathogen Taphrina deformans in asymptomatic leaves of peach (Prunus persica (L.) Batsch) in Russia

被引：0

作者：

Mikhailova, E., V ^{[1
]}

Karpun, N. N. ^{[1
]}

Agumava, A. A. ^{[1
,2
]}

Efremov, A. M. ^{[1
]}

Ravish, Choudhary ^{[3
]}

Samarina, L. S. ^{[1
]}

机构：

[1] Russian Acad Sci, Fed Res Ctr Subtrop Sci Ctr, Yana Fabritsiusa 2-28, Soci 354002, Russia

[2] Sci Res Inst Med Primatol, Fed State Budgetary Sci Inst, Soci, Russia

[3] ICAR IARI, Div Seed Sci & Technol, New Delhi 110012, India

来源：

RESEARCH JOURNAL OF BIOTECHNOLOGY | 2020年 / 15卷 / 09期

关键词：

Prunus persica; leaf curl; primers; Taphrina deformans; FUNGI;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Leaf curl disease is one of the main constraints of peach production in the world. The efficiency of two pairs of primer to detect leaf curl pathogen Taphrina deformans in asymptomatic peach leaves was analyzed. The amplification was confirmed using primer pairs viz., NL1/NL4 and TDITS1/NL4. Universal fungal primers NL1 / NL4 resulting a clear amplicon of 900 bp. 83-85% identity to T. deformans genome was confirmed using sequencing of PCR product obtained by TDITS1/NL4 primers. The data obtained will be useful for quantitative and qualitative study of this fungal pathogen and the dynamics of its life cycle.

引用

页码：126 / 129

页数：4

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