EDC4 interacts with and regulates the dephospho-CoA kinase activity of CoA synthase

被引：18

作者：

Gudkova, Dania ^{[1
]}

Panasyuk, Ganna ^{[1
]}

Nemazanyy, Ivan ^{[1
]}

Zhyvoloup, Alexander ^{[2
]}

Monteil, Pascale ^{[2
]}

Filonenko, Valeriy ^{[1
]}

Gout, Ivan ^{[2
]}

机构：

[1] Inst Mol Biol & Genet, State Key Lab Mol & Cellular Biol, UA-03680 Kiev, Ukraine

[2] UCL, Dept Struct & Mol Biol, London WC1E 6BT, England

来源：

FEBS LETTERS | 2012年 / 586卷 / 20期

关键词：

CoA synthase; CoA; Processing bodies; EDC4; Decapping; Mitochondria; COENZYME-A; PROCESSING BODY; P-BODIES; BIOSYNTHESIS; LOCALIZATION; AUTOANTIBODIES; MITOCHONDRIA; PROTEIN; GE-1; LINK;

D O I：

10.1016/j.febslet.2012.08.033

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Coenzyme A synthase (CoAsy) is a bifunctional enzyme which facilitates the last two steps of Coenzyme A biogenesis in higher eukaryotes. Here we describe that CoAsy forms a complex with enhancer of mRNA-decapping protein 4 (EDC4), a central scaffold component of processing bodies. CoAsy/EDC4 complex formation is regulated by growth factors and is affected by cellular stresses. EDC4 strongly inhibits the dephospho-CoA kinase activity of CoAsy in vitro. Transient overexpression of EDC4 decreases cell proliferation, and further co-expression of CoAsy diminishes this effect. Here we report that EDC4 might contribute to regulation of CoA biosynthesis in addition to its scaffold function in processing bodies.

引用

页码：3590 / 3595

页数：6

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