Fucoxanthin attenuates fatty acid-induced lipid accumulation in FL83B hepatocytes through regulated Sirt1/AMPK signaling pathway

被引:62
作者
Chang, Yi-Hsien [1 ]
Chen, Ya-Ling [1 ]
Huang, Wen-Chung [2 ,3 ]
Liou, Chian-Jiun [3 ,4 ]
机构
[1] Chang Gung Univ Sci & Technol, Dept Nutr & Hlth Sci, 261 Wenhua 1st Rd, Taoyuan 33303, Taiwan
[2] Chang Gung Univ Sci & Technol, Coll Human Ecol, Res Ctr Food & Cosmet Safety, Res Ctr Chinese Herbal Med,Grad Inst Hlth Ind Tec, 261 Wenhua 1st Rd, Taoyuan 33303, Taiwan
[3] Chang Gung Mem Hosp, Dept Pediat, Div Allergy Asthma & Rheumatol, Taoyuan 33303, Taiwan
[4] Chang Gung Univ Sci & Technol, Res Ctr Chinese Herbal Med, Dept Nursing, 261 Wenhua 1st Rd, Taoyuan 33303, Taiwan
关键词
AMPK; Anti-obesity; Fucoxanthin; Lipogenesis; Lipolysis; LIVER-DISEASE; LIPOGENESIS; CELLS; ADIPOCYTES; SUPPRESSES; ACTIVATION; PROMOTION; OXIDATION; LIPOLYSIS; SREBP-1C;
D O I
10.1016/j.bbrc.2017.11.022
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The fucoxanthin, isolated from brown algae, was reported to have multiple biological functions to anti inflammation, anti-tumor, and ameliorated obesity in mice. In this study we investigated whether fucoxanthin could inhibit lipids accumulation in FL83B hepatocytes. FL83B cells were induced as fatty liver cell model by 0.5 mM oleic acid for 48 h, and treated with various concentration of fucoxanthin for 24 h. The results demonstrated that fucoxanthin significantly suppressed lipid accumulation and decreased lipid peroxidation in hepatocytes. Fucoxanthin could decrease lipogenesis-related transcription factor expression, including sterol regulatory element-binding proteins 1c and peroxisome proliferator-activated receptor gamma. It also reduced fatty acid synthase expression and increased adipose triglyceride lipase and the phosphorylation of hormone-sensitive lipase production for lipolysis. Furthermore, fucoxanthin significantly increased phosphorylation of AMP-activated protein kinase (AMPK), and decreased activity of acetyl-CoA carboxylase for regulating fatty acid synthesis. The results suggest that fucoxanthin is an effective marine nature compound for increasing lipolysis and inhibiting lipogenesis in oleic acid induced fatty liver cells through promoted Sirt1/AMPK pathway. (C) 2017 Elsevier Inc. All rights reserved.
引用
收藏
页码:197 / 203
页数:7
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