Involvement of the Bcl-2 Family Members in Pinus massoniana Bark Extract induced Apoptosis in HeLa Cells

被引：30

作者：

Ma, Hongling ^{[1
,2
]}

Lai, Fei ^{[1
,2
]}

Xie, Heng ^{[3
]}

Wang, Jinfa ^{[1
,2
]}

Wang, Hongbin ^{[1
,2
]}

机构：

[1] Sun Yat Sen Zhongshan Univ, Sch Life Sci, State Key Lab Biocontrol, Minist Educ, Guangzhou 510275, Guangdong, Peoples R China

[2] Sun Yat Sen Zhongshan Univ, Sch Life Sci, Key Lab Gene Engn, Minist Educ, Guangzhou 510275, Guangdong, Peoples R China

[3] Inst Songzhen Nutr Resource, Guangzhou 510645, Guangdong, Peoples R China

来源：

PHYTOTHERAPY RESEARCH | 2008年 / 22卷 / 11期

关键词：

apoptosis; Bax; HeLa; mitochondria; Pinus massoniana;

D O I：

10.1002/ptr.2496

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Pinus massoniana bark extract (PMBE) contains a variety of flavonoids whose antioxidant properties have been confirmed in vitro. This study was undertaken to evaluate the cytotoxic effects and the mechanism of cell death on the PMBE-treated human cervical cancer cell line, HeLa. PMBE treatment led to cell growth inhibition in a dose- and time-dependent manner, and PMBE-induced apoptosis was confirmed by DAPI staining, TUNEL assays and sub-G1 phase accumulation. Cell cycle was also arrested in G2/M phase. Immunoblotting analysis showed that cytochrome c was released, the protein expression of Bax was increased, the protein expression of Bcl-2 was down-regulated and caspase-9 and -3 were activated in PMBE-treated HeLa cells. Taken together, PMBE inhibited proliferation, induces apoptosis and causes cell cycle arrest in HeLa cells, indicating that PMBE may be a potential therapeutic agent for cancer. Copyright (C) 2008 John Wiley & Sons, Ltd.

引用

页码：1472 / 1476

页数：5

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