Nanoscale structure of protamine/DNA complexes for gene delivery

被引：17

作者：

Motta, Simona ^{[1
]}

Brocca, Paola ^{[1
]}

Del Favero, Elena ^{[1
]}

Rondelli, Valeria ^{[1
]}

Cantu, Laura ^{[1
]}

Amici, Augusto ^{[2
]}

Pozzi, Daniela ^{[3
]}

Caracciolo, Giulio ^{[3
]}

机构：

[1] Univ Milan, Dipartimento Biotecnol Med & Med Traslaz, I-20090 Segrate, MI, Italy

[2] Univ Camerino, Dipartimento Biosci & Biotecnol, I-62032 Camerino, MC, Italy

[3] Univ Roma La Sapienza, Dipartimento Med Mol, I-00161 Rome, Italy

来源：

APPLIED PHYSICS LETTERS | 2013年 / 102卷 / 05期

关键词：

SMALL-ANGLE SCATTERING; DNA-PROTAMINE; REENTRANT CONDENSATION; IN-VITRO; SIZE; THERAPY; SALT;

D O I：

10.1063/1.4790588

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

Understanding the internal packing of gene carriers is a key-factor to realize both gene protection during transport and de-complexation at the delivery site. Here, we investigate the structure of complexes formed by DNA fragments and protamine, applied in gene delivery. We found that complexes are charge-and size-tunable aggregates, depending on the protamine/DNA ratio, hundred nanometers in size. Their compactness and fractal structure depend on the length of the DNA fragments. Accordingly, on the local scale, the sites of protamine/DNA complexation assume different morphologies, seemingly displaying clumping ability for the DNA network only for shorter DNA fragments. (C) 2013 American Institute of Physics. [http://dx.doi.org/10.1063/1.4790588]

引用

页数：4

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