Spectroscopic investigation of the interaction of BSA with cationic surfactants

被引：41

作者：

Madaeni, Sayed S. ^{[1
]}

Rostami, Elham ^{[1
]}

机构：

[1] Razi Univ, Dept Chem Engn, Membrane Res Ctr, Kermanshah, Iran

来源：

CHEMICAL ENGINEERING & TECHNOLOGY | 2008年 / 31卷 / 09期

关键词：

bovine serum albumin (BSA); fluorophore; quencher; surfactants;

D O I：

10.1002/ceat.200700496

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

The interactions between dodecyltrimethylammonium bromide (DTAB), tetradecyltrimethylammonium bromide (TTAB), cetyltrimethylammonium bromide (CTAB), and hexadecylpyridinium chloride (HDPC) with bovine serum albumin (BSA) in an aqueous solution (pH = 7.0, 0.001 M HEPES buffer) were studied by fluorescence and circular dichroism (CD) measurements. These categories of surfactants were used to elucidate the effect of hydrophilic group and length of hydrophobic chain surfactant on the mechanism of binding to BSA. The result revealed that for all surfactants, at low concentrations, the Stern-Volmer plots have an upward curvature and in high concentrations, the quenching efficiency was decreased with increase in surfactant concentration. The activation energy of the interaction between cationic surfactants and BSA was measured. The results of CD show that the conformation of BSA has been changed in the presence of cationic surfactants.

引用

页码：1265 / 1271

页数：7

共 35 条

[1] Effect of pH on the conformation, interaction with membranes and hemolytic activity of sticholysin II, a pore forming cytolysin from the sea anemone Stichodactyla helianthus [J].