Disturbances in microbial skin recolonization and cutaneous immune response following allogeneic stem cell transfer

被引：5

作者：

Bayer, Nadine ^{[1
,2
]}

Hausman, Bela ^{[3
,4
,5
]}

Pandey, Ram Vinay ^{[1
]}

Deckert, Florian ^{[2
,6
]}

Gail, Laura-Marie ^{[1
,2
,7
]}

Strobl, Johanna ^{[1
,2
]}

Pjevac, Petra ^{[3
,4
,8
]}

Krall, Christoph ^{[9
]}

Unterluggauer, Luisa ^{[1
]}

Redl, Anna ^{[1
,2
]}

Bachmayr, Victoria ^{[1
]}

Kleissl, Lisa ^{[1
,7
]}

Nehr, Marion ^{[5
]}

Kirkegaard, Rasmus ^{[3
,4
,8
]}

Makristathis, Athanasios ^{[5
]}

Watzenboeck, Martin L. ^{[10
]}

Nica, Robert ^{[11
]}

Staud, Clement ^{[12
]}

Hammerl, Lukas ^{[1
]}

Wohlfarth, Philipp ^{[13
]}

Ecker, Rupert C. ^{[11
,14
]}

Knapp, Sylvia ^{[6
]}

Rabitsch, Werner ^{[13
]}

Berry, David ^{[3
,4
,8
]}

Stary, Georg ^{[1
,2
,7
]}

机构：

[1] Med Univ Vienna, Dept Dermatol, A-1090 Vienna, Austria

[2] Austrian Acad Sci, CeMM Res Ctr Mol Med, A-1090 Vienna, Austria

[3] Med Univ Vienna, Joint Microbiome Facil, A-1030 Vienna, Austria

[4] Univ Vienna, A-1030 Vienna, Austria

[5] Med Univ Vienna, Dept Lab Med, Div Clin Microbiol, A-1090 Vienna, Austria

[6] Med Univ Vienna, Dept Med 1, Lab Infect Biol, A-1090 Vienna, Austria

[7] Ludwig Boltzmann Inst Rare & Undiagnosed Dis, A-1090 Vienna, Austria

[8] Univ Vienna, Ctr Microbiol & Environm Syst Sci, Dept Microbiol & Ecosyst Sci, Div Microbial Ecol, A-1030 Vienna, Austria

[9] Med Univ Vienna, Ctr Med Stat Informat & Intelligent Syst, A-1090 Vienna, Austria

[10] Med Univ Vienna, Dept Biomed Imaging & Image Guided Therapy, Vienna, Austria

[11] TissueGnost GmbH, A-1020 Vienna, Austria

[12] Med Univ Vienna, Dept Surg, Div Plast & Reconstruct Surg, A-1090 Vienna, Austria

[13] Med Univ Vienna, Dept Internal Med 1, Hematopoiet Stem Cell Transplantat Unit, A-1090 Vienna, Austria

[14] Queensland Univ Technol, Fac Hlth, Sch Biomed Sci, Brisbane, Qld 4059, Australia

来源：

LEUKEMIA | 2022年 / 36卷 / 11期

基金：

奥地利科学基金会;

关键词：

VERSUS-HOST-DISEASE; SINGLE CELLS; RNA-SEQ; MANIFESTATIONS; IDENTIFICATION; COMMUNITIES; DIVERSITY; PROGRAM; PROJECT;

D O I：

10.1038/s41375-022-01712-z

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The composition of the gut microbiome influences the clinical course after allogeneic hematopoietic stem cell transplantation (HSCT), but little is known about the relevance of skin microorganisms. In a single-center, observational study, we recruited a cohort of 50 patients before undergoing conditioning treatment and took both stool and skin samples up to one year after HSCT. We could confirm intestinal dysbiosis following HSCT and report that the skin microbiome is likewise perturbed in HSCT-recipients. Overall bacterial colonization of the skin was decreased after conditioning. Particularly patients that developed acute skin graft-versus-host disease (aGVHD) presented with an overabundance of Staphylococcus spp. In addition, a loss in alpha diversity was indicative of aGVHD development already before disease onset and correlated with disease severity. Further, co-localization of CD45(+) leukocytes and staphylococci was observed in the skin of aGVHD patients even before disease development and paralleled with upregulated genes required for antigen-presentation in mononuclear phagocytes. Overall, our data reveal disturbances of the skin microbiome as well as cutaneous immune response in HSCT recipients with changes associated with cutaneous aGVHD.

引用

页码：2705 / 2714

页数：10

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