Effects of cooking temperature and time on the color, morphology, crystallinity, thermal properties, starch-lipid complexes formation and rheological properties of roux

Roux is an equal part mixture of flour and clarified butter cooked for a few minutes, and is the basic recipe for producing sauces. There is a lack of systematic studies regarding the effects of cooking temperature and time on the characteristics of roux. To this end, roux pasta (50:50 of wheat flour and clarified butter) was prepared to assess the effects of cooking time (1, 2 and 4 min) and temperature (80 and 100 degrees C) on physicochemical and morphological properties. Viscosity and viscoelastic moduli increased with both temperature and cooking time, an effect ascribed to partial gelatinization of starch granules. XRD analysis showed that starch granules underwent crystallinity reduction ( similar to 20-60%) with both cooking temperature and time. Deconvoluted FTIR indicated gradual reduction of short-range ordering with time caused by leaching out of amylose chains from starch granules, leading to the formation of amylose-lipid inclusion complexes as corroborated by DSC measurements. Also, it was found that the secondary structure of proteins was affected by cooking, with the fraction of beta-sheet aggregates increasing (similar to 35%) upon cooking time. Overall, the above results provided a basis for understanding the very complex physicochemical changes occurring during roux manufacture.