A Novel Galactoglucomannan Exopolysaccharide Produced by Oil Fermentation withPseudozymasp. SY16

被引：4

作者：

Kim, Kwang Nyeon ^{[1
]}

Lee, Yong Jae ^{[2
]}

Cho, Dae-Hyun ^{[2
]}

Ahn, Keug-Hyun ^{[2
]}

Hong, Jong-Eon ^{[3
]}

Park, Yong-Il ^{[4
,5
]}

Kim, Hee-Sik ^{[2
,3
,6
]}

机构：

[1] United Act Inc, R&D Ctr, Seoul 08381, South Korea

[2] Korea Res Inst Biosci & Biotechnol KRIBB, Cell Factory Res Ctr, Daejeon 34141, South Korea

[3] DKBio Ltd, Daejeon 34141, South Korea

[4] Catholic Univ Korea, Dept Biotechnol, Bucheon 14662, South Korea

[5] Catholic Univ Korea, Res Ctr Biopharmaceut Lead Mol GRRC, Bucheon 14662, South Korea

[6] Univ Sci & Technol UST, Environm Biotechnol, Daejeon 34113, South Korea

来源：

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | 2020年 / 25卷 / 05期

关键词：

exopolysaccharide; galactoglucomannan; Pseudozymasp; yeast; fermentation; SPRUCE PICEA-ABIES; GLYCOLIPID BIOSURFACTANT; EXTRACELLULAR PRODUCTION; BIOMASS;

D O I：

10.1007/s12257-020-0066-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Recently, the production of exopolysaccharide fromPseudozymasp. SY16, which is mannosylerythritol lipid-producing yeast, has been occasionally observed during industrial fermentations. Moreover, exopolysaccharides derived from microbes are in high demand for the production of biopolymers. Therefore, this study aimed to investigate the production and characteristics of the exopolysaccharide produced by this strain. In batch fermentations with this strain, the maximum production yield of exopolysaccharide (5.8 g/L) was obtained under olive oil supplementation as a carbon source with culture conditions of 30 degrees C, pH 8.0, and 600 rpm. Through a series of liquid chromatography steps, the exopolysaccharide was successfully purified, and its emulsifying activity was demonstrated. In addition, two-dimensional nuclear magnetic resonance analysis revealed that the exopolysaccharide is a galactoglucomannan with a novel and unique structure, consisting of galactose, glucose, and mannose in the ratio of 1:5:3.

引用

页码：742 / 748

页数：7

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