Isolation and characterization of twenty-four polymorphic microsatellite loci for the topical seagrass, Thalassia hemprichii

被引：7
|
作者：
Wainwright, Benjamin J. [1 ]
Arlyza, Irma S. [2 ]
Karl, Stephen A. [1 ]
机构：
[1] Univ Hawaii Manoa, Hawaii Inst Marine Biol, Kaneohe, HI 96744 USA
[2] Indonesian Inst Sci, Res Ctr Oceanog, Jakarta 14430, Indonesia
来源：
CONSERVATION GENETICS RESOURCES | 2013年 / 5卷 / 04期
关键词：
Connectivity; Gene flow; Indonesia; Conservation; ABUNDANCE; PRIMERS;
D O I：
10.1007/s12686-013-9937-1
中图分类号：
X176 [生物多样性保护];
学科分类号：
090705 ;
摘要：
Twenty-four polymorphic microsatellite loci were developed for the tropical seagrass, Thalassia hemprichii. Microsatellite enriched genomic libraries were constructed and subsequently sequenced using Pacific Biosciences SMRT technology. The 24 loci were characterized in 19 individuals from the Tukang Besi Islands, South East Sulawesi, Indonesia. Observed levels of heterozygosity ranged from 0.26 to 0.95 with a mean of 4.7 alleles per locus. No pairs of loci showed evidence of significant linkage disequilibrium and one locus significantly deviated from Hardy-Weinberg expectations.
引用
收藏
页码：939 / 941
页数：3
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