Analysis of amino acid and monoamine neurotransmitters and their metabolites in rat urine of Alzheimer's disease using in situ ultrasound-assisted derivatization dispersive liquid-liquid microextraction with UHPLC-MS/MS

被引:36
作者
Zhao, Xian-En [1 ,2 ]
He, Yongrui [1 ,2 ]
Li, Meng [1 ,2 ]
Chen, Guang [1 ,2 ]
Wei, Na [1 ,2 ]
Wang, Xiao [5 ]
Sun, Jing [3 ,4 ]
Zhu, Shuyun [1 ,2 ]
You, Jinmao [1 ,2 ]
机构
[1] Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China
[2] Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China
[3] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China
[4] Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xining 810001, Qinghai, Peoples R China
[5] Shandong Acad Sci, Shandong Anal & Test Ctr, Key Lab TCM Qual Control Technol, 19 Keyuan St, Jinan 250014, Peoples R China
基金
中国国家自然科学基金;
关键词
Neurotransmitter; In situ derivatization; Ultrasound-assisted dispersive; liquid-liquid microextraction; UHPLC-MS/MS; Alzheimer's diseases; Urine; TANDEM MASS-SPECTROMETRY; LC-MS/MS; SIMULTANEOUS QUANTIFICATION; MULTIPLE NEUROTRANSMITTERS; CHROMATOGRAPHY; BRAIN; DOPAMINE; MICRODIALYSIS; EXTRACTION; SAMPLES;
D O I
10.1016/j.jpba.2016.11.056
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Neurotransmitters (NTs) may play an important role in neuro degenerative disorders such as Alzheimer's disease (AD). In order to investigate the potential links, a new simple, fast, accurate and sensitive analytical method, based on in situ ultrasound-assisted derivatization dispersive liquid-liquid microex-traction (in situ UA-DDLLME) coupled with ultra high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS), has been developed and validated. The quantitation of amino acid neu-rotransmitters (AANTs) and monoamine neurotransmitters (MANTs) in urine of AD rats were performed in this work. The in situ UA-DDLLME procedure involved the rapid injection of the mixture of low toxic 4-bromoanisole (extractant) and acetonitrile (dispersant), which containing the new designed and synthesized 4'-carbonyl chloride rosamine (CCR) as derivatization reagent, into the aqueous phase of real sample and buffer. Under the selected conditions, the derivatization and microextraction of analytes were simultaneously completed within 1 min. Good linearity for each analyte (R > 0.992) was observed with low limit of detections (LODs, S/N > 3). Moreover, the proposed method was compared with direct detection or other reported methods, and the results showed that low matrix effects and good recoveries results were obtained in this work. Taken together, in situ UA-DDLLME coupled with UHPLC-MS/MS analysis was demonstrated to be a good method for sensitive, accurate and simultaneous monitoring of AANTs and MANTs. This method would be expected to be highly useful in AD diseases' clinical diagnostics and may have potential value in monitoring the efficacy of treatment. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:186 / 198
页数:13
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