Phosphoproteomics and molecular cardiology: Techniques, applications and challenges

被引：17
作者：
Sun, Zeyu [1 ]
Hamilton, Karyn L. [2 ,4 ]
Reardon, Kenneth F. [1 ,2 ,3 ]
机构：
[1] Colorado State Univ, Dept Chem & Biol Engn, Ft Collins, CO 80523 USA
[2] Colorado State Univ, Grad Program Cell & Mol Biol, Ft Collins, CO 80523 USA
[3] Colorado State Univ, Sch Biomed Engn, Ft Collins, CO 80523 USA
[4] Colorado State Univ, Dept Hlth & Exercise Sci, Ft Collins, CO 80523 USA
来源：
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY | 2012年 / 53卷 / 03期
关键词：
Phosphoproteomics; Molecular cardiology; Phosphorylation; Mass spectrometry; Phosphopeptide enrichment; PROTEIN-KINASE-C; HYDROPHILIC-INTERACTION CHROMATOGRAPHY; ELECTRON-TRANSFER DISSOCIATION; ION AFFINITY-CHROMATOGRAPHY; QUANTITATIVE PROTEOMIC ANALYSIS; SCALE PHOSPHORYLATION ANALYSIS; EPSILON SIGNALING COMPLEXES; ISCHEMIA-REPERFUSION INJURY; TANDEM MASS-SPECTROMETRY; SWITCHING HPLC ANALYSIS;
D O I：
10.1016/j.yjmcc.2012.06.001
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Protein phosphorylation has been widely documented as a key regulatory and signaling mechanism associated with many cardiac diseases. Recent advances in phosphoproteomic technologies such as phosphopeptide enrichment, novel mass spectrometry applications, and bioinformatic tools have resulted in high-throughput identification and quantitation of protein phosphorylation in a global manner. This review summarizes mainstream phosphoproteomic workflows and highlights the most recent applications of phosphoproteomics used in a range of molecular cardiology research. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：354 / 368
页数：15
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