Fludarabine and Cladribine Induce Changes in Surface Proteins on Human B-Lymphoid Cell Lines Involved with Apoptosis, Cell Survival, and Antitumor Immunity
Fludarabine and cladribine are purine analogues used to treat hematological malignancies. Alone or in combination with therapeutic antibodies, they are effective in treating patients with chronic lymphocytic leukemia and non-Hodgkin's lymphoma. However, the mechanisms of action of these drugs are not well understood. Plasma membrane proteins perform a variety of essential functions that can be affected by malignancy and perturbed by chemotherapy. Analysis of surface proteins may contribute to an understanding of the mechanisms of action of purine analogues and identify biomarkers for targeted therapy. The surface of human cells is rich in N-linked glycoproteins, enabling use of a hydrazide-coupling technique to enrich for glycoproteins, with iTRAQ labeling for quantitative comparison. A number of plasma membrane proteins on human leukemia and lymphoma cells were affected by treatment with a purine analogue, including decreases in CD22 (an adhesion and signaling molecule) and increases in CD205 (a "damaged cell marker") and CD80 and CD50 (T-cell interaction molecules). Purine analogues may affect B-cell receptor (BCR) signaling and costimulatory molecules, leading to multiple signals for apoptosis and cell clearance. Fludarabine and cladribine induce differential effects, with some cell survival proteins (ECE-1 and CD 100) more abundant after fludarabine treatment. Cell surface proteins induced by fludarabine and cladribine may be targets for therapeutic antibodies.
机构:
China Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
China Med Univ Hosp, Dept Internal Med, Div Hematol & Oncol, Taichung, TaiwanChina Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
Bai, Li-Yuan
Ma, Yihui
论文数: 0引用数: 0
h-index: 0
机构:
Ohio State Univ, Coll Pharm, Div Med Chem & Pharmacognosy, Columbus, OH 43210 USAChina Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
Ma, Yihui
Kulp, Samuel K.
论文数: 0引用数: 0
h-index: 0
机构:
Ohio State Univ, Coll Pharm, Div Med Chem & Pharmacognosy, Columbus, OH 43210 USAChina Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
Kulp, Samuel K.
Wang, Shu-Huei
论文数: 0引用数: 0
h-index: 0
机构:
Ohio State Univ, Dept Internal Med, Div Hematol & Oncol, Columbus, OH 43210 USAChina Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
Wang, Shu-Huei
Chiu, Chang-Fang
论文数: 0引用数: 0
h-index: 0
机构:
China Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
China Med Univ Hosp, Dept Internal Med, Div Hematol & Oncol, Taichung, TaiwanChina Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
Chiu, Chang-Fang
Frissora, Frank
论文数: 0引用数: 0
h-index: 0
机构:
Ohio State Univ, Dept Internal Med, Div Hematol & Oncol, Columbus, OH 43210 USAChina Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
Frissora, Frank
Mani, Rajeswaran
论文数: 0引用数: 0
h-index: 0
机构:
Ohio State Univ, Dept Internal Med, Div Hematol & Oncol, Columbus, OH 43210 USAChina Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
Mani, Rajeswaran
Mo, Xiaokui
论文数: 0引用数: 0
h-index: 0
机构:
Ohio State Univ, Ctr Biostat, Columbus, OH 43210 USAChina Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
Mo, Xiaokui
Jarjoura, David
论文数: 0引用数: 0
h-index: 0
机构:
Ohio State Univ, Ctr Biostat, Columbus, OH 43210 USAChina Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
Jarjoura, David
Byrd, John C.
论文数: 0引用数: 0
h-index: 0
机构:
Ohio State Univ, Dept Internal Med, Div Hematol & Oncol, Columbus, OH 43210 USAChina Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
Byrd, John C.
Chen, Ching-Shih
论文数: 0引用数: 0
h-index: 0
机构:
Ohio State Univ, Coll Pharm, Div Med Chem & Pharmacognosy, Columbus, OH 43210 USAChina Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
Chen, Ching-Shih
Muthusamy, Natarajan
论文数: 0引用数: 0
h-index: 0
机构:
Ohio State Univ, Dept Internal Med, Div Hematol & Oncol, Columbus, OH 43210 USAChina Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
机构:
Univ Sains Malaysia, Sch Med Sci, Dept Pathol, George Town, MalaysiaUniv Sains Malaysia, Sch Med Sci, Dept Pathol, George Town, Malaysia
Ch'ng, Ewe Seng
Kumanogoh, Atsushi
论文数: 0引用数: 0
h-index: 0
机构:
Osaka Univ, Microbial Dis Res Inst, Immunol Frontier Res Ctr, Dept Immunopathol, Suita, Osaka 565, JapanUniv Sains Malaysia, Sch Med Sci, Dept Pathol, George Town, Malaysia
机构:
China Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
China Med Univ Hosp, Dept Internal Med, Div Hematol & Oncol, Taichung, TaiwanChina Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
Bai, Li-Yuan
Ma, Yihui
论文数: 0引用数: 0
h-index: 0
机构:
Ohio State Univ, Coll Pharm, Div Med Chem & Pharmacognosy, Columbus, OH 43210 USAChina Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
Ma, Yihui
Kulp, Samuel K.
论文数: 0引用数: 0
h-index: 0
机构:
Ohio State Univ, Coll Pharm, Div Med Chem & Pharmacognosy, Columbus, OH 43210 USAChina Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
Kulp, Samuel K.
Wang, Shu-Huei
论文数: 0引用数: 0
h-index: 0
机构:
Ohio State Univ, Dept Internal Med, Div Hematol & Oncol, Columbus, OH 43210 USAChina Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
Wang, Shu-Huei
Chiu, Chang-Fang
论文数: 0引用数: 0
h-index: 0
机构:
China Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
China Med Univ Hosp, Dept Internal Med, Div Hematol & Oncol, Taichung, TaiwanChina Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
Chiu, Chang-Fang
Frissora, Frank
论文数: 0引用数: 0
h-index: 0
机构:
Ohio State Univ, Dept Internal Med, Div Hematol & Oncol, Columbus, OH 43210 USAChina Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
Frissora, Frank
Mani, Rajeswaran
论文数: 0引用数: 0
h-index: 0
机构:
Ohio State Univ, Dept Internal Med, Div Hematol & Oncol, Columbus, OH 43210 USAChina Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
Mani, Rajeswaran
Mo, Xiaokui
论文数: 0引用数: 0
h-index: 0
机构:
Ohio State Univ, Ctr Biostat, Columbus, OH 43210 USAChina Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
Mo, Xiaokui
Jarjoura, David
论文数: 0引用数: 0
h-index: 0
机构:
Ohio State Univ, Ctr Biostat, Columbus, OH 43210 USAChina Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
Jarjoura, David
Byrd, John C.
论文数: 0引用数: 0
h-index: 0
机构:
Ohio State Univ, Dept Internal Med, Div Hematol & Oncol, Columbus, OH 43210 USAChina Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
Byrd, John C.
Chen, Ching-Shih
论文数: 0引用数: 0
h-index: 0
机构:
Ohio State Univ, Coll Pharm, Div Med Chem & Pharmacognosy, Columbus, OH 43210 USAChina Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
Chen, Ching-Shih
Muthusamy, Natarajan
论文数: 0引用数: 0
h-index: 0
机构:
Ohio State Univ, Dept Internal Med, Div Hematol & Oncol, Columbus, OH 43210 USAChina Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
机构:
Univ Sains Malaysia, Sch Med Sci, Dept Pathol, George Town, MalaysiaUniv Sains Malaysia, Sch Med Sci, Dept Pathol, George Town, Malaysia
Ch'ng, Ewe Seng
Kumanogoh, Atsushi
论文数: 0引用数: 0
h-index: 0
机构:
Osaka Univ, Microbial Dis Res Inst, Immunol Frontier Res Ctr, Dept Immunopathol, Suita, Osaka 565, JapanUniv Sains Malaysia, Sch Med Sci, Dept Pathol, George Town, Malaysia