Micro-/Nano-sized hydroxyapatite directs differentiation of rat bone marrow derived mesenchymal stem cells towards an osteoblast lineage

被引：91
作者：
Huang, Yan [1 ]
Zhou, Gang [1 ,2 ]
Zheng, Lisha [1 ,2 ]
Liu, Haifeng [1 ,2 ]
Niu, Xufeng [1 ,2 ]
Fan, Yubo [1 ,2 ]
机构：
[1] Beihang Univ, Sch Biol Sci & Med Engn, Minist Educ, Key Lab Biomech & Mechanobiol, Beijing 100191, Peoples R China
[2] Beihang Univ Shenzhen, Res Inst, Shenzhen 518057, Peoples R China
来源：
NANOSCALE | 2012年 / 4卷 / 07期
基金：
中国国家自然科学基金;
关键词：
STROMAL CELLS; OSTEOGENIC DIFFERENTIATION; RAPID FORMATION; IN-VITRO; BIOMATERIALS; IRRADIATION; SURFACE; INSPIRATION; CRYSTALS; STRESS;
D O I：
10.1039/c2nr12072k
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Regenerative medicine consisting of cells and materials provides a new way for the repair and regeneration of tissues and organs. Nano-biomaterials are highlighted due to their advantageous features compared with conventional micro-materials. The aim of this study is to investigate the effects of micro-/nano-sized hydroxyapatite (mu/n-HA) on the osteogenic differentiation of rat bone marrow derived mesenchymal stem cells (rBMSCs). mu/n-HA were prepared by a microwave synthesizer and precipitation method, respectively. Different sizes of mu/n-HA were characterized by IR, XRD, SEM, TEM and co-cultured with rBMSCs. It was shown that rBMSCs expressed higher levels of osteoblast-related markers by n-HA than mu-HA stimulation. The size of HA is an important factor for affecting the osteogenic differentiation of rBMSCs. This provides a new avenue for mechanistic studies of stem cell differentiation and a new approach to obtain more committed differentiated cells.
引用
收藏
页码：2484 / 2490
页数：7
相关论文
共 41 条
[21]  
LI YB, 1994, J MATER SCI-MATER M, V5, P326
[22]   Rapid formation of hydroxyapatite nanostructures by microwave irradiation [J].
Liu, JB ;
Li, KW ;
Wang, H ;
Zhu, MK ;
Yan, H .
CHEMICAL PHYSICS LETTERS, 2004, 396 (4-6) :429-432
[23]  
LOCKLIN RM, 1995, CLIN ORTHOP RELAT R, P27
[24]   Enhancement of osteoblastic differentiation of mesenchymal stromal cells cultured by selective combination of bone morphogenetic protein-2 (BMP-2) and fibroblast growth factor-2 (FGF-2) [J].
Maegawa, Naoki ;
Kawamura, Kenji ;
Hirose, Motohiro ;
Yajima, Hiroshi ;
Takakura, Yoshinori ;
Ohgushi, Hajime .
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 2007, 1 (04) :306-313
[25]   A biodegradable and biocompatible gecko-inspired tissue adhesive [J].
Mahdavi, Alborz ;
Ferreira, Lino ;
Sundback, Cathryn ;
Nichol, Jason W. ;
Chan, Edwin P. ;
Carter, David J. D. ;
Bettinger, Chris J. ;
Patanavanich, Siamrut ;
Chignozha, Loice ;
Ben-Joseph, Eli ;
Galakatos, Alex ;
Pryor, Howard ;
Pomerantseva, Irina ;
Masiakos, Peter T. ;
Faquin, William ;
Zumbuehl, Andreas ;
Hong, Seungpyo ;
Borenstein, Jeffrey ;
Vacanti, Joseph ;
Langer, Robert ;
Karp, Jeffrey M. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (07) :2307-2312
[26]   Characterization of adhesion and differentiation markers of osteogenic marrow stromal cells [J].
Marom, R ;
Shur, I ;
Solomon, R ;
Benayahu, D .
JOURNAL OF CELLULAR PHYSIOLOGY, 2005, 202 (01) :41-48
[27]   Hydroxyapatite nano and microparticles: Correlation of particle properties with cytotoxicity and biostability [J].
Motskin, M. ;
Wright, D. M. ;
Muller, K. ;
Kyle, N. ;
Gard, T. G. ;
Porter, A. E. ;
Skepper, J. N. .
BIOMATERIALS, 2009, 30 (19) :3307-3317
[28]   Preparation of size-controlled hexagonal CdS nanocrystallites and the characteristics of their surface structures [J].
Murakoshi, K ;
Hosokawa, H ;
Saitoh, M ;
Wada, Y ;
Sakata, T ;
Mori, H ;
Satoh, M ;
Yanagida, S .
JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS, 1998, 94 (04) :579-586
[29]   Stem cell fate dictated solely by altered nanotube dimension [J].
Oh, Seunghan ;
Brammer, Karla S. ;
Li, Y. S. Julie ;
Teng, Dayu ;
Engler, Adam J. ;
Chien, Shu ;
Jin, Sungho .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (07) :2130-2135
[30]   Mechanobiology of mesenchymal stem cells and their use in cardiovascular repair [J].
Park, Jennifer S. ;
Huang, Ngan F. ;
Kurpinski, Kyle T. ;
Patel, Shyam ;
Hsu, Steve ;
Li, Song .
FRONTIERS IN BIOSCIENCE-LANDMARK, 2007, 12 :5098-5116
12345