Micro-/Nano-sized hydroxyapatite directs differentiation of rat bone marrow derived mesenchymal stem cells towards an osteoblast lineage

被引：89
作者：
Huang, Yan [1 ]
Zhou, Gang [1 ,2 ]
Zheng, Lisha [1 ,2 ]
Liu, Haifeng [1 ,2 ]
Niu, Xufeng [1 ,2 ]
Fan, Yubo [1 ,2 ]
机构：
[1] Beihang Univ, Sch Biol Sci & Med Engn, Minist Educ, Key Lab Biomech & Mechanobiol, Beijing 100191, Peoples R China
[2] Beihang Univ Shenzhen, Res Inst, Shenzhen 518057, Peoples R China
来源：
NANOSCALE | 2012年 / 4卷 / 07期
基金：
中国国家自然科学基金;
关键词：
STROMAL CELLS; OSTEOGENIC DIFFERENTIATION; RAPID FORMATION; IN-VITRO; BIOMATERIALS; IRRADIATION; SURFACE; INSPIRATION; CRYSTALS; STRESS;
D O I：
10.1039/c2nr12072k
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Regenerative medicine consisting of cells and materials provides a new way for the repair and regeneration of tissues and organs. Nano-biomaterials are highlighted due to their advantageous features compared with conventional micro-materials. The aim of this study is to investigate the effects of micro-/nano-sized hydroxyapatite (mu/n-HA) on the osteogenic differentiation of rat bone marrow derived mesenchymal stem cells (rBMSCs). mu/n-HA were prepared by a microwave synthesizer and precipitation method, respectively. Different sizes of mu/n-HA were characterized by IR, XRD, SEM, TEM and co-cultured with rBMSCs. It was shown that rBMSCs expressed higher levels of osteoblast-related markers by n-HA than mu-HA stimulation. The size of HA is an important factor for affecting the osteogenic differentiation of rBMSCs. This provides a new avenue for mechanistic studies of stem cell differentiation and a new approach to obtain more committed differentiated cells.
引用
收藏
页码：2484 / 2490
页数：7
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